Antibiotic resistance and plasmid profiling of Vibrio parahaemolyticus isolated from shrimp farms along the southwest coast of India

Chakraborty, Rekha D and Surendran, P K and Chakraborty, Kajal (2009) Antibiotic resistance and plasmid profiling of Vibrio parahaemolyticus isolated from shrimp farms along the southwest coast of India. World Journal of Microbiology and Biotechnology . pp. 2005-2012.

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Abstract

Shrimp, water, and sediment samples were collected from various shrimp farms located in and around Cochin. V. parahaemolyticus was identified by standard biochemical tests and plasmid profiling was carried out for the isolates. Susceptibility was tested against 15 antibiotics before and after the plasmid curing. Incidence of V. parahaemolyticus was found in 46% of the samples screened. Antibiogram studies showed, above 50% of the strains sensitive to chlorotetracycline, chloramphenicol and nitrofurantoin. Multiple antibiotic resistance (MAR) index was found to be 0.2. Total presumptive Vibrio parahaemolyticus count (TPVPC) and resistance to antibiotics was found to be more in sediment samples particularly in premonsoon season. Plasmid profiles of V. parahaemolyticus isolates revealed seven plasmids in the size range of 0.75, 1.2, 6.0, and 8.0 kb sizes and 3 plasmids above 10.0 kb. The MAR index suggests the low risk potential involved in consuming seafoods.

Item Type:Article
Uncontrolled Keywords:Vibrio parahaemolyticus; Farms; Antibiotics; Plasmid; Curing
Subjects:Aquaculture
Fish Biotechnology
Fish Diseases
Crustacean Fisheries
Divisions:CMFRI-Cochin > Marine Capture > Crustacean Fisheries
ID Code:8697
Deposited By:Arun Surendran
Deposited On:24 Aug 2011 16:33
Last Modified:24 Aug 2011 16:33

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