Tiger Prawn (Penaeus monodon) broodstock of Andaman Molecular Characterisation by RAPD Technique

Kumar, Pavel and Sethi, S N and Mahendran, V and Nivas, K and Sundar, Jai (2011) Tiger Prawn (Penaeus monodon) broodstock of Andaman Molecular Characterisation by RAPD Technique. Fishing Chimes, 31 (1). pp. 24-27.

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Abstract

Random amplified polymorphic DNA (RAPD) analysis was used to examine genetic variation in Tiger prawn, Penaeus monodon. Specimens of this prawn were collected from two different geographically separated locations in the Andaman Sea. A total of 30 samples of P. monodon individuals were investigated using nine random primers. The PCR amplification of template DNA produced a total of 26 scorable RAPD bands, having molecular weight ranging from approximately 500 to 5,000 bp of the nine primers tested, the maximum number of loci amplified was 5 with primer 3 and primer 4 followed by primer 1 which recorded 4 distinct scorable bands.

Item Type: Article
Uncontrolled Keywords: Tiger Prawn; Penaeus monodon; broodstock; Andaman; Molecular Characterisation; RAPD Technique
Subjects: Fish Biotechnology
Aquaculture
Crustacean Fisheries > Prawn and Prawn fisheries
Divisions: CMFRI-Madras (Chennai)
Depositing User: Arun Surendran
Date Deposited: 25 May 2011 06:13
Last Modified: 09 Sep 2015 15:49
URI: http://eprints.cmfri.org.in/id/eprint/8523

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