Mohindra, Vindhya and Gopalakrishnan, A and Rajaswaminathan, T and Paulton, M P and Sajeela, K A (2009) Isolation of total DNA. Training Manual on Advances in DNA Marker Techniques . pp. 32-45.
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Abstract
Total DNA can be extracted from fresh, frozen or fixed blood, fresh/frozen liver, muscle, sperms, kidney and any other tissue of fish. A slight modification of extraction protocol has to be done for each type as well as state of storage of tissue. Most frequently used method of DNA isolation is the phenol-chloroform method, which removes proteins and other cellular components from nucleic acids and relatively pure DNA can be obtained for further analysis.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Isolation; DNA |
| Subjects: | Fish Genetics |
| Divisions: | CMFRI-Cochin > Marine Biotechnology |
| ID Code: | 8420 |
| Deposited By: | Arun Surendran |
| Deposited On: | 10 May 2011 13:37 |
| Last Modified: | 10 May 2011 13:37 |
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