Isolation of total DNA

Mohindra, Vindhya and Gopalakrishnan, A and Rajaswaminathan, T and Paulton, M P and Sajeela, K A (2009) Isolation of total DNA. Training Manual on Advances in DNA Marker Techniques . pp. 32-45.

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Abstract

Total DNA can be extracted from fresh, frozen or fixed blood, fresh/frozen liver, muscle, sperms, kidney and any other tissue of fish. A slight modification of extraction protocol has to be done for each type as well as state of storage of tissue. Most frequently used method of DNA isolation is the phenol-chloroform method, which removes proteins and other cellular components from nucleic acids and relatively pure DNA can be obtained for further analysis.

Item Type:Article
Uncontrolled Keywords:Isolation; DNA
Subjects:Fish Genetics
Divisions:CMFRI-Cochin > Marine Biotechnology
ID Code:8420
Deposited By:Arun Surendran
Deposited On:10 May 2011 13:37
Last Modified:10 May 2011 13:37

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