Isolation of total DNA

Mohindra, Vindhya and Gopalakrishnan, A and Rajaswaminathan, T and Paulton, M P and Sajeela, K A (2009) Isolation of total DNA. Training Manual on Advances in DNA Marker Techniques. pp. 32-45.

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Total DNA can be extracted from fresh, frozen or fixed blood, fresh/frozen liver, muscle, sperms, kidney and any other tissue of fish. A slight modification of extraction protocol has to be done for each type as well as state of storage of tissue. Most frequently used method of DNA isolation is the phenol-chloroform method, which removes proteins and other cellular components from nucleic acids and relatively pure DNA can be obtained for further analysis.

Item Type: Article
Uncontrolled Keywords: Isolation; DNA
Subjects: Fish Genetics
Divisions: CMFRI-Cochin > Marine Biotechnology
Depositing User: Arun Surendran
Date Deposited: 10 May 2011 08:07
Last Modified: 09 Sep 2015 15:49

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