Isolation of total DNA

Mohindra, Vindhya and Gopalakrishnan, A and Raja Swaminathan, T and Paulton, M P and Sajeela, K A (2009) Isolation of total DNA. Training Manual on Advances in DNA Marker Techniques. pp. 32-45.

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Abstract

Total DNA can be extracted from fresh, frozen or fixed blood, fresh/frozen liver, muscle, sperms, kidney and any other tissue of fish. A slight modification of extraction protocol has to be done for each type as well as state of storage of tissue. Most frequently used method of DNA isolation is the phenol-chloroform method, which removes proteins and other cellular components from nucleic acids and relatively pure DNA can be obtained for further analysis.

Item Type:	Article
Uncontrolled Keywords:	Isolation; DNA
Subjects:	Fish Genetics
Divisions:	CMFRI-Kochi > Marine Biotechnology, Fish Nutrition and Health Division Subject Area > CMFRI > CMFRI-Kochi > Marine Biotechnology, Fish Nutrition and Health Division CMFRI-Kochi > Marine Biotechnology, Fish Nutrition and Health Division Subject Area > CMFRI-Kochi > Marine Biotechnology, Fish Nutrition and Health Division
Depositing User:	Arun Surendran
Date Deposited:	10 May 2011 08:07
Last Modified:	15 Feb 2023 06:08
URI:	http://eprints.cmfri.org.in/id/eprint/8420

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