Polymerase Chain Reaction

Thomas, P C (2009) Polymerase Chain Reaction. Training Manual on Advances in DNA Marker Techniques. pp. 46-58.

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Polymerase Chain Reaction or PCR is defined as a molecular technique wbich allow in Vitro synthe is of billions of copies of a target DNA fragment within several hour from a DNA template using a simple enzymatic reaction by performing successive rounds of in vitro nucleic acid replication withou a living organism, such as E. coli or yeast This is achieved by using two oligonucleotide primers that hybridize (annealing) to the opposite strand of the target DNA at positions that flank the region to be amplified through sinllll, aneous extension of both primers.

Item Type: Article
Uncontrolled Keywords: Polymerase Chain Reaction; PCR
Subjects: Fish Biotechnology
Fish Genetics
Divisions: CMFRI-Cochin > Marine Biotechnology
Depositing User: Arun Surendran
Date Deposited: 10 May 2011 08:06
Last Modified: 09 Sep 2015 15:49
URI: http://eprints.cmfri.org.in/id/eprint/8417

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