Gene cloning - techniques and strategies

Abstract

Genetic information is coded in DNA found in the nucleus and mitochondria of animal cells and chloroplast of plant cells. The flow of genetic information from DNA to RNA and subsequently to polypeptides or proteins forms the central dogma of molecular biology. From the complex pool of DNA how can one isolate and study a specific sequence, or in other words, a gene of interest? The technology of gene cloning allows the amplification and recovery of a specific fragment from the highly complex DNA pool so that it can ,be comprehensively studied with regard to its structure, function, regulation, expression, etc