Designing of primer for PCR

Thomas, P C (2003) Designing of primer for PCR. In: Winter school on Recent Advances in Mariculture Genetics and Biotechnology, 4- 24 November 2003, Cochin.


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The polymerase chain reaction (PCR) is the technique for in vitro synthesis of multiple copies of a given DNA molecule. Primers are the most important components of PCR and success of PCR largely depends on the primers. A primer is an oligonucleotide. It is utilized as the starting point for the synthesis of the DNA fragment in a PCR. Primers bind to its complementary sequences on the DNA template and extend in length by addition of nucleotide to its 3' end. Amplification of specific regions of the template DNA depends on the design of primer sequences.

Item Type: Conference or Workshop Item (Paper)
Uncontrolled Keywords: Designing of primer; PCR
Subjects: Fish Genetics
Divisions: CMFRI-Cochin > Marine Biotechnology
Depositing User: Arun Surendran
Date Deposited: 05 Mar 2011 12:19
Last Modified: 09 Sep 2015 15:46

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