The polymerase chain reaction

Thomas, P C and Paulton, M P (2003) The polymerase chain reaction. In: Winter school on Recent Advances in Mariculture Genetics and Biotechnology, 4- 24 November 2003, Cochin.

[img]
Preview
PDF
7Mb

Abstract

The Polymerase Chain Reaction (PCR) is a technique for the in vitro synthesis of billions of copies of a specific nucleic acid sequence by performing successive rounds of in vitro nucleic acid replication. This is achieved by using two oligonucleotide primers that hybridize (annealing) to the opposite strand of the target DNA at positions that flank the region to be amplified through simultaneous extension of both primers.

Item Type:Conference or Workshop Item (Paper)
Uncontrolled Keywords:polymerase chain reaction
Subjects:Biochemistry
Divisions:CMFRI-Cochin > Marine Biotechnology
CMFRI-Cochin > Mariculture
ID Code:7862
Deposited By:Arun Surendran
Deposited On:05 Mar 2011 17:50
Last Modified:05 Mar 2011 17:50

Repository Staff Only: item control page