Pathobiological studies of Vibrio alginolyticus biofilm on the defense functions and disease resistance in Giant tiger shrimp (Penaeus monodon)

Sharma, S R Krupesha (2008) Pathobiological studies of Vibrio alginolyticus biofilm on the defense functions and disease resistance in Giant tiger shrimp (Penaeus monodon). ["eprint_fieldopt_thesis_type_phd" not defined] thesis, Karnataka Veterinary, Animal and Fisheries Sciences, Bidar.

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Abstract

The present study was undertaken to develop and evaluate the biofIlm of Vibrio alginolyticus for oral immunostimulation in tiger shrimp, Penaeus monodon. Development of biofIlm of V. alginolyticus was standardized and maximum bioftlm formation was achieved with 0.15 per cent TSB supplemented with 2 per cent NaCI on 0 .3 per cent chitin flakes with incubation for 3 d. The biofllm cell population was inversely related to the planktonic cell population during the culture. The biofllm cells could be inactivated at 800C and 10 per cent formalin while free cells at 600C and 0.5 per cent formalin suggesting the resistant nature of bioftlm to action by heat and chemicals. In SDS-PAGE, biofllm cells showed an increased expression of 3 new proteirts and repression of 4 proteins compared to free cells. Elevated values of the immune parameters with respect to total haemocyte count, phenol oxidase activity, total haemolymph protein and antibacterial activity were observed in shrimps exposed to 108 , 109 and 1010 cfu/g shrimp/ day bioftlm cells when compared to control shrimps. The values were highest in 109 and 1010 cfu groups with no significant difference between them. It was observed that 109 cfu/ g shrimp/day for 14 days was the optimum dose and time required to activate the immune system of juvenile P. monodon.

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