Diwan, A D and Joseph, Shoji (2000) Cryopreservation of spermatophores of the marine shrimp Penaeus indicus H. Milne Edwards. Journal of Aquaculture in Tropics, 15 (1). pp. 35-43.
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Abstract
Attempts on cryopreservation of spermatozoa have been made in penaeid shrimp Penaeus indicus. Viable spermatozoa was preserved successfully for a period of 60 days at -3S'C and -196'C temperatures. Among several cryoprotectants used, a combination of DMSO (5%) and glycerol (5%), and DMSO (5%) and trehalose (O.25M) showed best viability rate (75 to 80%) in freeze thawed spermatozoa. Activational changes in spermatozoa during acrosome reaction after induction with egg water have been mentioned. The importance of cryogenic storage of spermatozoa is discussed.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Cryopreservation; spermatophores;e marine shrimp; Penaeus indicus |
| Subjects: | Fish Biotechnology Crustacean Fisheries > Prawn and Prawn fisheries |
| Divisions: | CMFRI-Cochin > Physiology and Nutrition Pathology |
| ID Code: | 7382 |
| Deposited By: | Arun Surendran |
| Deposited On: | 08 Feb 2011 15:19 |
| Last Modified: | 08 Feb 2011 15:19 |
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