Diwan, A D and Joseph, Shoji (2000) Cryopreservation of spermatophores of the marine shrimp Penaeus indicus H. Milne Edwards. Journal of Aquaculture in Tropics, 15 (1). pp. 35-43.
Attempts on cryopreservation of spermatozoa have been made in penaeid shrimp Penaeus indicus. Viable spermatozoa was preserved successfully for a period of 60 days at -3S'C and -196'C temperatures. Among several cryoprotectants used, a combination of DMSO (5%) and glycerol (5%), and DMSO (5%) and trehalose (O.25M) showed best viability rate (75 to 80%) in freeze thawed spermatozoa. Activational changes in spermatozoa during acrosome reaction after induction with egg water have been mentioned. The importance of cryogenic storage of spermatozoa is discussed.
|Uncontrolled Keywords:||Cryopreservation; spermatophores;e marine shrimp; Penaeus indicus|
Crustacean Fisheries > Prawn and Prawn fisheries
|Divisions:||CMFRI-Cochin > Physiology and Nutrition Pathology
Subject Areas > CMFRI Brochures > CMFRI-Cochin > Physiology and Nutrition Pathology
|Depositing User:||Arun Surendran|
|Date Deposited:||08 Feb 2011 09:49|
|Last Modified:||09 Sep 2015 15:43|
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