Cryopreservation of spermatophores of the marine shrimp Penaeus indicus H. Milne Edwards

Diwan, A D and Joseph, Shoji (2000) Cryopreservation of spermatophores of the marine shrimp Penaeus indicus H. Milne Edwards. Journal of Aquaculture in Tropics, 15 (1). pp. 35-43.

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Abstract

Attempts on cryopreservation of spermatozoa have been made in penaeid shrimp Penaeus indicus. Viable spermatozoa was preserved successfully for a period of 60 days at -3S'C and -196'C temperatures. Among several cryoprotectants used, a combination of DMSO (5%) and glycerol (5%), and DMSO (5%) and trehalose (O.25M) showed best viability rate (75 to 80%) in freeze thawed spermatozoa. Activational changes in spermatozoa during acrosome reaction after induction with egg water have been mentioned. The importance of cryogenic storage of spermatozoa is discussed.

Item Type: Article
Uncontrolled Keywords: Cryopreservation; spermatophores;e marine shrimp; Penaeus indicus
Subjects: Fish Biotechnology
Crustacean Fisheries > Prawn and Prawn fisheries
Divisions: CMFRI-Cochin > Physiology and Nutrition Pathology
Subject Areas > CMFRI Brochures > CMFRI-Cochin > Physiology and Nutrition Pathology
Depositing User: Arun Surendran
Date Deposited: 08 Feb 2011 09:49
Last Modified: 09 Sep 2015 15:43
URI: http://eprints.cmfri.org.in/id/eprint/7382

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