Nutritional studies on sebae Anemonefish, Amphiprion sebae Bleeker 1853, with special reference To protein and lipid requirements

Binu, Varghese (2004) Nutritional studies on sebae Anemonefish, Amphiprion sebae Bleeker 1853, with special reference To protein and lipid requirements. PhD thesis, Central Institute of Fisheries Education, Mumbai.

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Abstract

Anemonefishes or clownfishes command higher price than most other pomacentrids in the marine aquarium fish trade. Commercial production of these reef fishes can generate valuable foreign exchange for the country. The sebae anemonefisi1, Amphiprion sebae Bleeker 1853, is one of the popular marine aquarium fish . At present rearing of the fish relies heavily on live and fresh diets, and formulated diets are seldom used due to inadequate knowledge of the nutritional needs of the different life-history stages. The present study focuses on the nutrition of broodstock, larvae and juvenile stages of the fish in captivity. In order to identify suitable diets for brood fishes the efficacy of six natural and five formulated diets was determined. The natural diets tested were deep sea shrimp, cuttlefish, squid, mature mussel, mussel meat, and a mixed diet of polychaete worm and mussel meat (1 :1). Among the natural diets cuttlefish meat gave significantly higher number of eggs per spawning (1521±264 eggs; mean ± SO, n=9) followed by the deep sea shrimp (1300±445 eggs). Among Ihe five formulated semi-moist diets used, diet CBOs with about 50% protein and 10% lipid, gave significantly higher number (2137±110) of eggs pHr spawning followed by diet CB02 (1683±436 eggs) which had 40% protein and 20% lipid. The number of eggs per spawning was found to decrease significantly when broodfish were fed ad libitum once a day (416128 eggs) compared to those fed ad libitum twice daily (885±55 eggs). Hatchery reared larvae weaned to a dry diet from 3rd week onwards matured and the broodfish spawned successfully in aquaria from 15th month (age of fish) onwards. Feeding experiments using exclusive or combinations of rotifers, Artemia, and Moina showed better survival when the larvae were reared with micro algae and fed rotifers and Artemia. Weaning studies revealed that weaning from livefeed to a dry microbound particulate diet after 3 weeks (21dph) did not Significantly affect survival, though the best age of weaning was found to be 30 days post hatching. Experiments with a purified and semi-purified diet having 50% protein and 6% lipid showed semi-purified diets to be better than purified diets. Analysis of data from protein requirement experiments using semi-purified isocaloric diets showed 46.2% as protein requirement for maximum weight gain in juveniles and 44.9% in terms of SGR. Lipid requirement experiment on juveniles using five isoproteic semi-purified diets showed 10.96% as lipid requirement for maximum weight gain and 11 .05% in terms of SGR. The present study proved the effectiveness of formulated diets in rearing larvae, juveniles and brood stock of the clownfish. The results are significant with enormous application in commercial production of these high value fishes and in promoting indigenous aquarium feed manufacture.

Item Type:Thesis (PhD)
Supervisor(s):Paulraj, R
Uncontrolled Keywords:Nutritional studies; sebae; Anemonefish; Amphiprion sebae; protein; lipid
Subjects:Fish Biotechnology
Theses
Aquaculture > Ornamental Fishes
Divisions:CMFRI-Cochin > Mariculture
ID Code:7221
Deposited By:Dr. V Mohan
Deposited On:31 Jan 2011 16:06
Last Modified:31 Jan 2011 16:06

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