Isolation and culture of protoplasts from agar yielding seaweed Gracilaria edulis (Gmelin) Silva

Kaladharan, P and Gopinathan, C P and Bindhu, K B and Smitha, K S and Gireesh, R (2000) Isolation and culture of protoplasts from agar yielding seaweed Gracilaria edulis (Gmelin) Silva. Seaweed Research and Utilisation, 22 (1&2). pp. 61-65.

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Abstract

Viable protoplasts werl! isokn~d [rom cuJt ure~ thallus of ag<J.r y~ilding red ::)~aweed GI"Gciiarill eduhs using an cnzymc mixture of 3% ONOZUKt"\ R-IO cdlulase 3% R-\ 0 maceroz),lll c, 1% agarasc and 1% pectinase in a seawr!cd protoplast washing mediulI1 (SPW) containing seawater and 1 M mannitol. After repeated washing wiih sterile ml:dium, the pro~op l il $IS were maintained in light and attempts were made on eJ..::ctrofllsioll of protopl;J$ts.

Item Type:Article
Uncontrolled Keywords:protoplasts; agar yielding; seaweed culture; Gracilaria edulis; (Gmelin) Silva
Subjects:Algae > Seaweed
Divisions:CMFRI-Cochin > Fishery Environment
ID Code:7044
Deposited By:Dr. V Mohan
Deposited On:20 Jan 2011 14:33
Last Modified:09 Mar 2011 14:56

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