Kaladharan, P and Gopinathan, C P and Bindhu, K B and Smitha, K S and Gireesh, R (2000) Isolation and culture of protoplasts from agar yielding seaweed Gracilaria edulis (Gmelin) Silva. Seaweed Research and Utilisation, 22 (1&2). pp. 61-65.
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Abstract
Viable protoplasts werl! isokn~d [rom cuJt ure~ thallus of ag<J.r y~ilding red ::)~aweed GI"Gciiarill eduhs using an cnzymc mixture of 3% ONOZUKt"\ R-IO cdlulase 3% R-\ 0 maceroz),lll c, 1% agarasc and 1% pectinase in a seawr!cd protoplast washing mediulI1 (SPW) containing seawater and 1 M mannitol. After repeated washing wiih sterile ml:dium, the pro~op l il $IS were maintained in light and attempts were made on eJ..::ctrofllsioll of protopl;J$ts.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | protoplasts; agar yielding; seaweed culture; Gracilaria edulis; (Gmelin) Silva |
| Subjects: | Algae > Seaweed |
| Divisions: | CMFRI-Kochi > Fishery Environment Management Division Subject Area > CMFRI > CMFRI-Kochi > Fishery Environment Management Division CMFRI-Kochi > Fishery Environment Management Division Subject Area > CMFRI-Kochi > Fishery Environment Management Division |
| Depositing User: | Dr. V Mohan |
| Date Deposited: | 20 Jan 2011 09:03 |
| Last Modified: | 09 Sep 2015 15:41 |
| URI: | http://eprints.cmfri.org.in/id/eprint/7044 |
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