Potential application of RAPD and RAHM markers in genome analysis of scombroid fishes

Jayasankar, P and Dharmalingam, K (1997) Potential application of RAPD and RAHM markers in genome analysis of scombroid fishes. Current Science, 72 (6). pp. 383-390.

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Abstract

Total genomic DNA from 30 different specimens of Indian mackerel Rastrelliger kanagurta (Cuvier) and 5 king seer Scomberomorus commerson (Lacepede) was extracted by a simple modified phenolchloroform protocol. PCR amplification conditions using 10-mer arbitrary primers were optimized. Primers with the same nucleotide sequences produced varying Random amplified polymorphic DNA (RAPD) patterns and those with different sequences generated different banding patterns for the same template in the two commercially important scombroid fishes.

Item Type:Article
Uncontrolled Keywords:Potential application; RAPD; RAHM; genome analysis; scombroid fishes
Subjects:Pelagic Fisheries > Scombridae
Fish Genetics
Divisions:CMFRI-Cochin > Marine Biotechnology
ID Code:6891
Deposited By:Arun Surendran
Deposited On:12 Jan 2011 16:25
Last Modified:12 Jan 2011 16:39

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