Phenotypic and molecular typing of Vibrio harveyi isolates and their pathogenicity to tiger shrimp larvae

Alavandi, S V and Manoranjita, V and Vijayan, K K and Kalaimani, N and Santiago, T C (2006) Phenotypic and molecular typing of Vibrio harveyi isolates and their pathogenicity to tiger shrimp larvae. Letters in Applied Microbiology, 43 . pp. 566-570.

[img]PDF
Restricted to CMFRI users only, others may use->

121Kb

Abstract

The objective of the present study was to identify the biotype(s) and molecular type(s) of Vibrio harveyi associated with pathogenicity in tiger shrimp (Penaeus monodon) larvae. Five luminescent and four nonluminescent V. harveyi isolates were subjected to phenotyping and random amplified polymorphic DNA (RAPD) fingerprinting, and pathogenicity testing to P. monodon mysis. Four isolates induced 34–41% mortality of P. monodon mysis when challenged at the rate of 106 CFU ml)1 within 60 h. Sucrose-fermenting biotypes of V. harveyi appeared to be associated with pathogenicity to larval shrimp. Higher temperature and salinity appeared to play a role on the onset of vibriosis and mortality in the challenged larval shrimp. Pathogenic isolates of V. harveyi could be demarcated as revealed by their clustering in the dendrogram constructed based on the RAPD fingerprints.

Item Type:Article
Uncontrolled Keywords:molecular typing; Vibrio harveyi; pathogenicity; tiger shrimp larvae
Subjects:Eggs and Larvae
Crustacean Fisheries > Prawn and Prawn fisheries
Divisions:CMFRI-Cochin > Marine Capture > Crustacean Fisheries
ID Code:6835
Deposited By:Arun Surendran
Deposited On:14 Dec 2010 18:04
Last Modified:16 Jul 2012 14:55

Repository Staff Only: item control page