Phenotypic and molecular typing of Vibrio harveyi isolates and their pathogenicity to tiger shrimp larvae

Alavandi, S V and Manoranjita, V and Vijayan, K K and Kalaimani, N and Santiago, T C (2006) Phenotypic and molecular typing of Vibrio harveyi isolates and their pathogenicity to tiger shrimp larvae. Letters in Applied Microbiology, 43. pp. 566-570.

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    Abstract

    The objective of the present study was to identify the biotype(s) and molecular type(s) of Vibrio harveyi associated with pathogenicity in tiger shrimp (Penaeus monodon) larvae. Five luminescent and four nonluminescent V. harveyi isolates were subjected to phenotyping and random amplified polymorphic DNA (RAPD) fingerprinting, and pathogenicity testing to P. monodon mysis. Four isolates induced 34–41% mortality of P. monodon mysis when challenged at the rate of 106 CFU ml)1 within 60 h. Sucrose-fermenting biotypes of V. harveyi appeared to be associated with pathogenicity to larval shrimp. Higher temperature and salinity appeared to play a role on the onset of vibriosis and mortality in the challenged larval shrimp. Pathogenic isolates of V. harveyi could be demarcated as revealed by their clustering in the dendrogram constructed based on the RAPD fingerprints.

    Item Type: Article
    Uncontrolled Keywords: molecular typing; Vibrio harveyi; pathogenicity; tiger shrimp larvae
    Subjects: Eggs and Larvae
    Crustacean Fisheries > Prawn and Prawn fisheries
    Divisions: CMFRI-Kochi > Marine Capture > Crustacean Fisheries
    Subject Area > CMFRI Brochures > CMFRI-Kochi > Marine Capture > Crustacean Fisheries
    CMFRI-Kochi > Marine Capture > Crustacean Fisheries
    Depositing User: Arun Surendran
    Date Deposited: 14 Dec 2010 12:34
    Last Modified: 09 Sep 2015 15:40
    URI: http://eprints.cmfri.org.in/id/eprint/6835

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