Billard, R and Bieniarz, K and Popek, W and Epler, P and Breton, B and Alagarswami, K (1987) Stimulation of gonadotropin secretion and spermiation in carp by pimozide-LRH-A treatment: Effects of dose and time of day. Aquaculture, 62 (2). pp. 161-170.Full text not available from this repository. (Request a copy)
To define the optimal combination time and LRH-A (luteinizing releasing hormone analogue) administration route for inducing spermiation in carp, two experiments were carried out. In the first experiment, carp kept in raceways in running recycled water in August (at a water temperature of 15–20°C) were injected intraperitoneally with pimozide (PIM) at 1 or 10 mg/kg body weight as well as with Des-Gly10 [D-Tryp6] LH-RH-ethylamide (LRH-A) at 0.1, 1 or 10 μg/kg; these products were given 6 h apart. The response was evaluated by the volume of milt and the number of spermatozoa emitted and by the gonadotropin (GTH) concentration in the plasma. At doses of 0.1, 1 and 10 μg/kg LRH-A, the total milt production was increased 2, 4 and 6-fold respectively when these treatments were combined with 1 mg PIM. In the second experiment, adult male carp, held in cages in a pond in a natural environment (at a water temperature of 11–14°C) at the beginning of May, received an intraperitoneal injection of PIM (1 mg/kg body weight) and a silicone rubber implant of LRH-A (Des-Gly10 [D-Ala6] - LH-RH-ethylamide) at dose of 10 μg/kg body weight simultaneously. Other groups of fish received PIM plus a blank implant. All of the treatments were given at either 6 a.m. or 8 p.m. When given at 6 a.m., the treatment induced a similar rise in plasma GTH (12–15 ng/ml) 24 h later in the groups treated with PIM and LRH-A implant alone or in combination. When the same treatment was given at 8 p.m., no response was observed 24 or 48 h later. Spermiation was not stimulated in any of the groups.
|Uncontrolled Keywords:||gonadotropin secretion; spermiation; carp; pimozide-LRH-A treatment; dose; time of day|
|Subjects:||Aquaculture > Induced Breeding|
|Depositing User:||Users 171 not found.|
|Date Deposited:||10 Nov 2010 05:38|
|Last Modified:||10 Nov 2010 05:38|
Actions (login required)