Electrophoretic separation of proteins

Subhashini, M H and Ravindranath, M H (1981) Electrophoretic separation of proteins. CMFRI Special Publication (7). pp. 103-116.


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There are several structural and storage proteins in the tissues of Crustacea. Some of them are metallo-proteins, some are enzymic in nature. Several electrophoretic procedures were used for the separation of these proteins. The methods used by crustacean workers include paper (Zuckerkandl, 1956; Hughes & Winkler, 1966 ; Stewart, et al., 1969), agar gel (Decleir, 1961; Vranckx &Durlait, 1976), starch gel (Whittaker, 1959 ; Cowden & Coleman, 1962; Dall, 1964) and polyacrylamide gel electrophoresis (Dall, 1974; Durliat et al., 1975; Alikhan & Akthar, 1980). With polyacrylamide gel electrophoresis a good resolution is achieved with minimum quantity of blood sample (0.1 ml). Here polyacrylamide gel electrophoretic method of Davis (1964) is described.

Item Type: Article
Uncontrolled Keywords: Electrophoretic separation; proteins; Crustacean Biochemistry and Physiology
Subjects: Crustacean Fisheries
Fish and Fisheries > Biochemical Study
Divisions: Contributors
CMFRI-Cochin > Marine Capture > Crustacean Fisheries
Subject Areas > CMFRI Brochures > CMFRI-Cochin > Marine Capture > Crustacean Fisheries
Depositing User: Geetha P Mrs
Date Deposited: 24 Sep 2010 06:07
Last Modified: 09 Sep 2015 15:21
URI: http://eprints.cmfri.org.in/id/eprint/3152

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