Electrophoretic separation of proteins

Abstract

There are several structural and storage proteins in the tissues of Crustacea. Some of them are metallo-proteins, some are enzymic in nature. Several electrophoretic procedures were used for the separation of these proteins. The methods used by crustacean workers include paper (Zuckerkandl, 1956; Hughes & Winkler, 1966 ; Stewart, et al., 1969), agar gel (Decleir, 1961; Vranckx &Durlait, 1976), starch gel (Whittaker, 1959 ; Cowden & Coleman, 1962; Dall, 1964) and polyacrylamide gel electrophoresis (Dall, 1974; Durliat et al., 1975; Alikhan & Akthar, 1980). With polyacrylamide gel electrophoresis a good resolution is achieved with minimum quantity of blood sample (0.1 ml). Here polyacrylamide gel electrophoretic method of Davis (1964) is described.