Thomas, P C and Mallia, Jyothi V and Muthiah, P (2004) Triploidy induction and confirmation in the edible oyster Crassosfrea madrasensis. Journal of the Marine Biological Association of India, 46 (2). pp. 224-228.
This paper presents the protocols standardized for producing triploid edible oyster, Crassostrea madrasensis by retention of I1 polar body (MI1 triploids), using physical (heat and cold shocks) and chemical agents (Cytochalasin B and 6-Dimethylaminopurine), as well as the protocol for preparation of metaphase spreads for cytological confirmation of triploidy and estimation of its percentage. Among the four inducing agents tried, 6-Dimethylaminopurine yielded highest percentage of triploids (66.6 in day old and 61.82 in "D" stage larvae).
|Uncontrolled Keywords:||Triploidy induction; edible oyster; Crassosfrea madrasensis|
|Subjects:||Molluscan Fisheries > Edible oyster|
|Divisions:||CMFRI-Cochin > Physiology and Nutrition Pathology|
|Deposited By:||Edwin Joseph|
|Deposited On:||05 Aug 2010 17:55|
|Last Modified:||12 Jul 2011 16:28|
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