Ultrastructural changes in the spermatozoa of the goldspot mullet Liza parsia (Hamilton-Buchanan) in different diluents during cryopreservation

Sukumaran, Sandhya and Noble, D and Gopalakrishnan, A and Sanil, N K (2003) Ultrastructural changes in the spermatozoa of the goldspot mullet Liza parsia (Hamilton-Buchanan) in different diluents during cryopreservation. Journal of the Marine Biological Association of India, 45 (1). pp. 47-53.

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Abstract

The milt of goldspot mullet, Liza parsia (Hamilton-Buchanan) was diluted with four extenders containing 10% DMSO. Samples were collected at four critical steps of cryopreservation procedure. Percentage of intact spermatozoa and cryoinjuries at each step was recorded. Extender V2E in seawater base +lo% DMSO appeared to be the best cryodiluent as the sperms exhibited least structural changes during the cryopreservation protocol. Chao's extender+lO% DMSO was the next preferred cryodiluent. The suitability of various extenders that accorded protection to injuries was evaluated on the basis of electron microscopic images.

Item Type:Article
Uncontrolled Keywords:Spermatozoa; Goldspot mullet; Liza parsia (Hamilton-Buchanan); Cryopreservation
Subjects:Demersal Fishes > Mullet
Divisions:CMFRI-Cochin > Marine Capture > Demersal Fisheries
ID Code:2003
Deposited By:Edwin Joseph
Deposited On:10 Aug 2010 16:25
Last Modified:10 Aug 2010 16:25

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