In vitro and in silico analysis unravelled clinically desirable attributes of Bacillus altitudinis L-asparaginase

Lailaja, V P and Hari, Vishnu and Sumithra, T G and Nair, V Anusree and Suresh, Gayathri and Sanil, N K and Sharma, S R Krupesha and Gopalakrishnan, A (2024) In vitro and in silico analysis unravelled clinically desirable attributes of Bacillus altitudinis L-asparaginase. Journal of Applied Microbiology, 135 (3). pp. 1-17. ISSN 1365-2672

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Abstract

Marine bacterial strains (number = 105) isolated from marine crabs were evaluated through a stepwise strategy incorporating the crucial attributes for therapeutic safety. The results demonstrated the potential of eight bacterial species for extracellular L-asparaginase production. However, only one isolate (Bacillus altitudinis CMFRI/Bal-2) showed clinically desirable attributes, viz. extracellular production, type-II nature, lack of concurrent L-glutaminase and urease activities, and presence of ansZ (functional gene for clinical type). The enzyme production was 22.55 ± 0.5 µM/mg protein/min within 24 h without optimization. The enzyme also showed good activity and stability in pH 7–8 and temperature 37°C, predicting the functioning inside the human body. The Michealis-Menten constant (Km) was 14.75 µM. Detailed in silico analysis based on functional gene authenticating the results of in vitro characterization and predicted the nonallergenic characteristic of the candidate. Docking results proved the higher affinity of the shortlisted candidate to L-asparagine than L-glutamine and urea.

Item Type:	Article
Uncontrolled Keywords:	anticancer drug; L-glutaminase; Urease; ansB; 3D homology model; molecular docking
Subjects:	Algae > Bacteria Biochemistry
Divisions:	CMFRI-Kochi > Marine Biotechnology, Fish Nutrition and Health Division Subject Area > CMFRI > CMFRI-Kochi > Marine Biotechnology, Fish Nutrition and Health Division CMFRI-Kochi > Marine Biotechnology, Fish Nutrition and Health Division Subject Area > CMFRI-Kochi > Marine Biotechnology, Fish Nutrition and Health Division
Depositing User:	Arun Surendran
Date Deposited:	18 Apr 2024 05:37
Last Modified:	18 Apr 2024 05:37
URI:	http://eprints.cmfri.org.in/id/eprint/18277

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