Pharmacological potential of seaweed-associated heterotrophic bacterium Bacillus atrophaeus

Varghese, Chesvin and Chakraborty, Kajal and Asharaf, Sumayya (2023) Pharmacological potential of seaweed-associated heterotrophic bacterium Bacillus atrophaeus. Archives of Microbiology, 205 (10). pp. 1-12. ISSN 1432-072X

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Abstract

Extremities in marine environmental conditions led the marine macroalga-associated bacteria to adapt and biosynthesize potential bioactive agents. The myriad of marine macroalgae and the bacterial flora they are associated with constitute a potential source of bioactive components with significant biotechnological and pharmacological applications. Heterotrophic bacteria associated with the intertidal macroalgae were isolated and assessed for their pharmacological properties. Subsequently, Firmicutes dominated more than half of the 152 cultivable isolates from macroalgae-associated bacteria collected from the Gulf of Mannar (9°17′0′′ N, 79°7′0′′ E), on Peninsular India’s southern coast. A total of 43 of those demonstrated steady antibacterial activities against a wide range of nosocomial pathogens. Among the bacteria isolated from marine macroalgae, Bacillus atrophaeus SHB2097 (MW821482) exhibited significant antimicrobial activities against clinically important pathogens. Organic extract of B. atrophaeus SHB2097 showed potential antimicrobial activities against test pathogens (minimum inhibitory concentration 6.25 µg/mL). Organic extract of B. atrophaeus SHB2097 revealed promising inhibition potential against cyclooxygenase-2 (IC90 53.26 µg/mL) and 5-lipoxygenase (IC90 9.74 µg/mL). The carbolytic enzyme α-glucosidase inhibition potential of the organic extract of the studied heterotrophic bacterium was significantly greater than (IC90 118 µg/mL) than that displayed by acarbose (IC90 645 µg/mL, p < 0.05). The significance of nuclear magnetic resonance-centered analyses of distinguishing signals in the organic extract and correlating those with bioactive potential was accentuated. The utilities of nuclear magnetic resonance-based fingerprinting emphasized the assessment of the distinctive signals in the solvent extracts and their correlation with the pharmacological properties. Thus, the heterotrophic B. atrophaeus SHB2097 could be used to develop potential therapeutic and biomedical agents.

Item Type:	Article
Uncontrolled Keywords:	Macroalga-associated heterotrophic bacteria; Bacillus atrophaeus SHB2097; Pharmacological properties; Antimicrobial; Anti-inflammatory
Subjects:	Biochemistry Algae > Seaweed
Divisions:	CMFRI-Kochi > Marine Biotechnology, Fish Nutrition and Health Division Subject Area > CMFRI > CMFRI-Kochi > Marine Biotechnology, Fish Nutrition and Health Division CMFRI-Kochi > Marine Biotechnology, Fish Nutrition and Health Division Subject Area > CMFRI-Kochi > Marine Biotechnology, Fish Nutrition and Health Division
Depositing User:	Arun Surendran
Date Deposited:	05 Dec 2022 04:16
Last Modified:	04 Jan 2024 07:21
URI:	http://eprints.cmfri.org.in/id/eprint/16504

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