Co-infection of Lactococcus garvieae and Tilapia lake virus (TiLV) in Nile tilapia O. niloticus cultured in India

Swaminathan, T R and Nithianantham, Sundar Raj and Narendrakumar, Lekshmi and Darmaratnam, Arathi and Sood, Neeraj and Pradhan, P K and Sharma, S R Krupesha and Lal, Kuldeep Kumar (2021) Co-infection of Lactococcus garvieae and Tilapia lake virus (TiLV) in Nile tilapia O. niloticus cultured in India. Diseases of Aquatic Organisms (DAO), 147. pp. 127-140.

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Official URL: https://www.int-res.com/abstracts/dao/v147/p127-14...
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Abstract

Tilapia lake virus (TiLV) and Lactococcus garvieae are 2 major pathogens of cultured Nile tilapia Oreochromis niloticus. In June-July 2018, a disease outbreak was reported in Nile tilapia cultured in brackish water floating cages in Kerala, India. Affected fish died gradually, and cumulative mortality reached ~75% within 1 mo. In the present study, TiLV and L. garvieae were isolated from the infected fish and confirmed. Nucleotide analysis of the partial sequence of segment 3 revealed that the present TiLV isolate showed 100% similarity with TiLV MF574205 and 97.65% similarity with TiLV KU552135 isolated in Israel. The partial 16S rDNA nucleotide sequence of L. garvieae shared 99% similarity with the 16S rDNA nucleotide sequence of L. garvieae isolated from Nile tilapia in Brazil. Eight virulence genes (hly1, hly2, hly3, NADH oxidase, adhPav, LPxTG-1, LPxTG-4, adhC1) were amplified in the present isolate. In the experimental challenge study, the onset of mortality started earlier in fish co-infected with TiLV and L. garvieae (3 d post-infection [dpi]) compared to other groups. Cumulative mortality (90% at 12 dpi) was significantly higher in the co-infected group than in fish infected with TiLV (60% at 12 dpi) and L. garvieae (40% at 12 dpi) alone. This study reveals that synergistic co-infection with TiLV and other bacteria may increase mortality in disease outbreaks. To the best of our knowledge, this is the first reported co-infection of L. garvieae with TiLV associated with mass mortality in Nile tilapia in India.

Item Type: Article
Uncontrolled Keywords: Co-infection; India; Lactococcus garvieae; Nile tilapia; TiLV; Tilapia lake virus.
Subjects: Aquaculture > Tropical fish
Fish and Fisheries > Fish Disease
Fish and Fisheries > Fish Pathology
Divisions: CMFRI-Kochi > Marine Biotechnology
Subject Area > CMFRI > CMFRI-Kochi > Marine Biotechnology
CMFRI-Kochi > Marine Biotechnology
Subject Area > CMFRI-Kochi > Marine Biotechnology
Depositing User: Arun Surendran
Date Deposited: 23 Mar 2022 08:31
Last Modified: 23 Mar 2022 08:31
URI: http://eprints.cmfri.org.in/id/eprint/15859

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