Optimization of media and culture conditions and changes in the levels of major biochemical constituents during growth stages of Nannochloropsis oculata in the laboratory

Joseph, Reena V and Saramma, A V and Kaladharan, P and Vijayalakshmi, K and Muralidharan, Abhijith and Anil, P and Jifriya, M J (2021) Optimization of media and culture conditions and changes in the levels of major biochemical constituents during growth stages of Nannochloropsis oculata in the laboratory. Journal of the Marine Biological Association of India, 63 (2). pp. 31-35.

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Abstract

Comparison of media and a few culture conditions for growing Nannochloropsis oculata, in laboratory is the major objective of this communication. Axenic stock culture was inoculated to different culture media and were evaluated for biomass production and pigment content. Of the three media studied, the f/2 medium was found to produce maximum cell density. Culture conditions like pH, temperature and salinity, optimized in f/2 medium revealed that pH 9, temperature 20°C and salinity 25 ppt were found to yield optimum output. Estimation of biochemical constituents showed more lipid content in the stationary phase, indicating the right harvesting time and this live feed organisms can also be considered for biofuel in the future

Item Type:	Article
Uncontrolled Keywords:	Live feed organism; log phase; chlorophyll a; PUFA; biofuel
Subjects:	Algae > Algal nutrition Biochemistry Algae
Divisions:	CMFRI-Kochi > Fishery Environment Management Division Subject Area > CMFRI > CMFRI-Kochi > Fishery Environment Management Division CMFRI-Kochi > Fishery Environment Management Division Subject Area > CMFRI-Kochi > Fishery Environment Management Division
Depositing User:	Arun Surendran
Date Deposited:	19 Feb 2022 08:58
Last Modified:	19 Feb 2022 08:58
URI:	http://eprints.cmfri.org.in/id/eprint/15800

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