Characterisation of vitellogenin and vitellin of Fenneropenaeus merguiensis (De Man)

Zacharia, Sherly and Kakati, V S (2004) Characterisation of vitellogenin and vitellin of Fenneropenaeus merguiensis (De Man). Indian Journal of Fisheries, 51 (3). pp. 255-263.

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    Abstract

    Purification and characterisation of vitellogenin and vitellin from haemolymph and mature ovaries respectively of Fenneropenaeus merguiensis were conducted using ammonium sulphate precipitation technique. The vitellogenin level in the haemolymph was low in the early stages of development, but increased significantly in the late maturing stages (Stage III) and fell abruptly in spent females. Purified vitellogenin had an approximate molecular weight of 350kDa as determined by PAGE and was characterised as lipoglycoprotein with calcium affinity on the basis of staining data. Two forms of purified vitellin (Vn1 and Vn2) of molecular weights 550 and 300kDa were also isolated. Vn1 was characterised as a lipo-glyco-calcium complex while Vn2 was glycoprotein in nature based on staining data. SDS PAGE analysis revealed 6 subunits in purified vitellogenin and 5 subunits in vitellin.

    Item Type: Article
    Uncontrolled Keywords: vitellogenin; vitellin; fenneropenaeus merguiensis
    Subjects: Crustacean Fisheries
    Divisions: CMFRI-Kochi > Marine Capture > Crustacean Fisheries
    Subject Area > CMFRI Brochures > CMFRI-Kochi > Marine Capture > Crustacean Fisheries
    CMFRI-Kochi > Marine Capture > Crustacean Fisheries
    Depositing User: Users 5 not found.
    Date Deposited: 15 Mar 2010 12:38
    Last Modified: 09 Sep 2015 15:05
    URI: http://eprints.cmfri.org.in/id/eprint/158

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