Leap Forward towards Unraveling Newer Anti‑infective Agents from an Unconventional Source: a Draft Genome Sequence Illuminating the Future Promise of Marine Heterotrophic Bacillus sp. Against Drug‑Resistant Pathogens

Chakraborty, Kajal and Vinaya, K K and Joy, Minju and Chakraborty, Rekha D (2021) Leap Forward towards Unraveling Newer Anti‑infective Agents from an Unconventional Source: a Draft Genome Sequence Illuminating the Future Promise of Marine Heterotrophic Bacillus sp. Against Drug‑Resistant Pathogens. Marine Biotechnology. pp. 1-19.

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Official URL: https://link.springer.com/article/10.1007%2Fs10126...
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Abstract

During the previous decade, genome-built researches on marine heterotrophic microorganisms displayed the chemical heterogeneity of natural product resources coupled with the efcacies of harnessing the genetic divergence in various strains. Herein, we describe the whole genome data of heterotrophic Bacillus amyloliquefaciens MB6 (MTCC 12,716), isolated from a marine macroalga Hypnea valentiae, a 4,107,511-bp circular chromosome comprising 186 contigs, with 4154 protein-coding DNA sequences and a coding ratio of 86%. Simultaneously, bioactivity-guided purifcation of the bacterial extract resulted in six polyketide classes of compounds with promising antibacterial activity. Draft genome sequence of B. amyloliquefaciens MB6 unveiled biosynthetic gene clusters (BGCs) engaged in the biosynthesis of polyketide–originated macrolactones with prospective antagonistic activity (MIC≤5 µg/mL) against nosocomial pathogens. Genome analysis manifested 34 putative BGCs necessitated to synthesize biologically active polyketide-originated frameworks or their derivatives. These results provide insights into the genetic basis of heterotrophic B. amyloliquefaciens MTCC 12,716 as a prospective lead for biotechnological and pharmaceutical applications

Item Type: Article
Uncontrolled Keywords: Marine heterotrophic Bacillus amyloliquefaciens MB6; Anti-infective polyketide metabolites; Biosynthetic gene clusters; Whole-genome data; Macrocyclic lactones; Drug-resistant pathogens
Subjects: Biochemistry > Bioactive compounds
Biochemistry
Algae
Divisions: CMFRI-Kochi > Marine Biotechnology
Subject Area > CMFRI > CMFRI-Kochi > Marine Biotechnology
CMFRI-Kochi > Marine Biotechnology
Subject Area > CMFRI-Kochi > Marine Biotechnology
Depositing User: Arun Surendran
Date Deposited: 23 Sep 2021 06:39
Last Modified: 23 Sep 2021 06:39
URI: http://eprints.cmfri.org.in/id/eprint/15346

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