Valorisation of black carrot pomace: microwave assisted extraction of bioactive phytoceuticals and antioxidant activity using Box–Behnken design

Kumar, Manoj and Dahuja, Anil and Sachdev, Archana and Kaur, Charanjith and Varghese, Eldho and Saha, Supradip and Sairam, K V S S (2019) Valorisation of black carrot pomace: microwave assisted extraction of bioactive phytoceuticals and antioxidant activity using Box–Behnken design. Journal of Food Science and Technology, 56 (1). pp. 995-1007.

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Official URL: https://link.springer.com/article/10.1007/s13197-0...
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    Abstract

    The present study compares three methods viz. microwave assisted extraction (MAE), ultrasonic-assisted extraction (UAE) and conventional solvent extraction (CSE) for extraction of phenolic compounds from black carrot pomace (BCP). BCP is the major by-product generated during processing and poses big disposal problem. Box–Behnken design using response surface methodology was employed to investigate and optimize the MAE of phenolics, antioxidant activity and colour density from BCP. The conditions for maximum recovery of polyphenolics were: microwave power (348.07 W), extraction time (9.8 min), solvent–solid ratio (19.3 mL/g) and ethanol concentration (19.8%). Under these conditions, the extract contained total phenolic content of 264.9 ± 10.02 mg gallic acid equivalents (GAE)/100 mL, antioxidant capacity (AOC) of 13.14 ± 1.05 lmol Trolox equivalents (TE)/mL and colour density of 68.63 ± 5.40 units. The total anthocyanin content at optimized condition was 753.40 ± 31.6 mg/L with low % polymeric colour of 7.40 ± 0.42. At optimized conditions, MAE yielded higher colour density (68.63 ± 5.40), polyphenolic content (264.9 ± 10.025 mg GAE/100 mL) and AOC (13.14 ± 1.05 lmol TE/mL) in a short time as compared to UAE and CSE. Overall results clearly indicate that MAE is the best suited method for extraction in comparison to UAE and CSE. The phenolic rich extract can be used as an effective functional ingredient in foods.

    Item Type: Article
    Uncontrolled Keywords: Bioactive content; Antioxidant capacity; Degradation kinetics; Chromacity; Hyper and bathochromic shifts
    Subjects: Biochemistry > Bioactive compounds
    Biochemistry
    Divisions: CMFRI-Kochi > Marine Capture > Fishery Resource Assessment
    Subject Area > CMFRI > CMFRI-Kochi > Marine Capture > Fishery Resource Assessment
    CMFRI-Kochi > Marine Capture > Fishery Resource Assessment
    Subject Area > CMFRI-Kochi > Marine Capture > Fishery Resource Assessment
    Depositing User: Arun Surendran
    Date Deposited: 06 Feb 2021 08:20
    Last Modified: 06 Feb 2021 08:20
    URI: http://eprints.cmfri.org.in/id/eprint/14845

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