Coinfection with two strains of Photobacterium damselae subsp. damselae and Vibrio harveyi in cage farmed cobia, Rachycentron canadum (Linnaeus, 1766)

Sharma, S R Krupesha and Dube, Praveen and Silpa, P S and Mini, K G and Pradeep, M A and Sanil, N K (2020) Coinfection with two strains of Photobacterium damselae subsp. damselae and Vibrio harveyi in cage farmed cobia, Rachycentron canadum (Linnaeus, 1766). Aquaculture Research.

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    Abstract

    In the case of coinfections, multiple pathogens induce pathologies independently or in combination. The present study describes the pathological and microbiological characterization of Vibrio harveyi and two strains of Photobacterium damselae subsp. damselae causing coinfection in cobia reared in sea cages. All the isolated pathogens were virulent to juvenile cobia by injection challenge. The experimental challenge with the three isolated pathogens singly or in combination revealed a significant difference in the mortality profile. Lesions in natural coinfection consisted of ascites, fibrinous deposits on internal organs, and increased melanomacrophage centres (MMC) and extensive necrosis in kidneys. Lesions in experimental infection were limited to congestion of internal organs. Enzymatic activity of both extracellular products (ECP) and live cells by well diffusion showed the highest enzymatic index for gelatinase in the ECP of V. harveyi. The enzyme activity evaluated based on the API‐ZYM system revealed the highest activity for alkaline and acid phosphatase in both ECP and bacteria. The MAR index indicated that the pathogens were from a low‐risk region of antibiotic contamination.

    Item Type: Article
    Uncontrolled Keywords: cobia, coinfection, experimental challenge, Photobacterium damselae subsp. damselae, Vibrio harveyi
    Subjects: Aquaculture > Cage culture
    Demersal Fishes > Cobia
    Fish Biotechnology > Microbiology
    Aquaculture
    Fish Biotechnology
    Divisions: CMFRI-Karwar
    CMFRI-Kochi > Marine Biotechnology
    Subject Area > CMFRI > CMFRI-Kochi > Marine Biotechnology
    CMFRI-Kochi > Marine Biotechnology
    Subject Area > CMFRI-Kochi > Marine Biotechnology

    CMFRI-Kochi > Marine Capture > Fishery Resource Assessment
    Subject Area > CMFRI > CMFRI-Kochi > Marine Capture > Fishery Resource Assessment
    CMFRI-Kochi > Marine Capture > Fishery Resource Assessment
    Subject Area > CMFRI-Kochi > Marine Capture > Fishery Resource Assessment
    Depositing User: Arun Surendran
    Date Deposited: 01 Dec 2020 10:29
    Last Modified: 01 Dec 2020 10:29
    URI: http://eprints.cmfri.org.in/id/eprint/14721

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