Analytical validation of a modified turbidimetric assay to screen sulphur oxidizing bacteria

Amala, P V and Sumithra, T G and Reshma, K J and Anju, F and Subramanniyan, Shinoj and Vijayagopal, P (2020) Analytical validation of a modified turbidimetric assay to screen sulphur oxidizing bacteria. Journal of Microbiological Method, 176. pp. 1-6.

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    Abstract

    Conventional turbidimetric assay for sulphate determination was modified to 100 times lesser reaction volume on a convenient format using microtitre plate based platform, targeting routine microbiological applications to screen sulphur oxidizing bacteria (SOB) cultures. The modified assay was linear up to 1500 mg/L of sulphate concentration, which is about 37.5 times more than that of conventional assay. Upon regression analysis, linear equation y = 1.243× + 0.011 was obtained having R2 value of 0.998. The modified assay was fully validated in terms of precision, limit of detection (LOD), limit of quantification (LOQ), sensitivity, selectivity and robustness to assure the reliability during final applications. LOD and LOQ were found as 7.4 mg/L and 24.8 mg/L of sulphate concentration respectively. Further, accuracy of the assay over routine SOB screening media components was tested, and proved as reliable and suitable for the intended application.

    Item Type: Article
    Uncontrolled Keywords: SOB; Thiosulphate; Sulphate concentration; Calibration curve; Spectrometry; Microtitre plate
    Subjects: Algae > Bacteria
    Fish Biotechnology > Microbiology
    Fish Biotechnology
    Divisions: CMFRI-Kochi > Marine Biotechnology
    Subject Area > CMFRI > CMFRI-Kochi > Marine Biotechnology
    CMFRI-Kochi > Marine Biotechnology
    Subject Area > CMFRI-Kochi > Marine Biotechnology

    CMFRI - Krishi Vigyan Kendra (KVK)
    Depositing User: Arun Surendran
    Date Deposited: 03 Nov 2020 12:33
    Last Modified: 03 Nov 2020 12:33
    URI: http://eprints.cmfri.org.in/id/eprint/14717

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