Transcriptomic approach to study salinity tolerance in euryhaline cichlid, Etroplus suratensis (Bloch, 1790)

Arun Kumar, T V and Pradeep, M A and Arshad, Esha and Vijayan, K K (2020) Transcriptomic approach to study salinity tolerance in euryhaline cichlid, Etroplus suratensis (Bloch, 1790). Journal of the Marine Biological Association of India, 62 (1). pp. 24-32.

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    Abstract

    Increasing levels of salinity in coastal and freshwater environments as a result of rise in sea level is linked to climate change and it impacts fish growth adversely. Etroplus suratensis (Pearlspot) is the largest species among the indigenous cichlid which is essentially brackishwater fish that has become naturally acclimated to freshwater. Understanding the basis of tolerance and acclimatization to increased environmental salinity and freshwater condition can help the regional aqua-farmers to mitigate predicted impacts of climate change in that region. Technically the last decade contributed many methodologies to understand the salinity adaptation mechanisms; suppression subtractive hybridization (SSH) library generation being one of them. In this study we performed SSH technique to identify genes involved in salinity adaptation and osmoregulation in gills of E. suratensis, generating from fishes exposed to seawater (SW-36 ‰) as compared to freshwater (FW-0 ‰). Here, SSH was used to identify alterations in gene transcription of E. suratensis after exposure to 36‰. A random 105 clones were selected and sanger sequenced from the subtractive c-DNA libraries which gave a total of 80 gene fragment sequences.

    Item Type: Article
    Uncontrolled Keywords: Euryhaline; salinity tolerance; SSH; c-DNA library; gene expression profile
    Subjects: Climate Change
    Marine Environment
    Divisions: CMFRI-Kochi > Marine Biotechnology
    Subject Area > CMFRI > CMFRI-Kochi > Marine Biotechnology
    CMFRI-Kochi > Marine Biotechnology
    Subject Area > CMFRI-Kochi > Marine Biotechnology
    Depositing User: Mr. Prashanth P K
    Date Deposited: 12 Oct 2020 10:09
    Last Modified: 12 Oct 2020 10:09
    URI: http://eprints.cmfri.org.in/id/eprint/14684

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