Polygalactan from bivalve Crassostrea madrasensis attenuates nuclear factor- κB activation and cytokine production in lipopolysaccharide-activated macrophage

Chakraborty, Kajal and Krishnan, Soumya and Joy, Minju (2020) Polygalactan from bivalve Crassostrea madrasensis attenuates nuclear factor- κB activation and cytokine production in lipopolysaccharide-activated macrophage. Carbohydrate Polymers, 249. pp. 1-11.

[img] Text
Carbohydrate Polymers_2020_Kajal Chakraborty_Polygalactan from bivalve Crassostrea madrasensis.pdf
Restricted to Registered users only

Download (4MB) | Request a copy
Official URL: https://www.sciencedirect.com/science/article/pii/...
Related URLs:

    Abstract

    A polygalactosamino-glucopyranosyl fucopyranose →4)-β-GlcAp{(3→1)-α-Fucp}-β-GalNAcp-(4,6-SO3−)-(1→ isolated from the bivalve Crassostrea madrasensis exhibited prospective anti-inflammatory activity against cyclooxygenase-2 and 5-lipoxygenase (IC50 < 50 μg mL−1) on lipopolysaccharide-induced macrophages. The polygalactan attenuated inducible nitric oxide synthase (IC50 65.7 μg mL−1) in lipopolysaccharide-prompted inflammation leading to the reduction of pro-inflammatory cytokine nitric oxide (236.2 μg mL−1 lysate), nuclear factor-κB, tumor necrosis factor-α, and interleukins (0.19−0.22 units mg−1 protein at 100 μg mL-1) by inhibiting cyclooxygenase-2. The polygalacatan suppressed the mRNA of nuclear factor-κB and cyclooxygenase-2 in lipopolysaccharide-induced macrophages. Western blot experiment revealed that the polygalactan attenuated the migration of nuclear factor-κB-p65 to the nucleus from cytoplasm, and suppressed the phosphorylation of α-subunit of κB inhibitor. Greater selectivity index of sulfated polygalactan (3.93) towards inducible cyclooxygenase-2 as compared with the anti-inflammatory agent ibuprofen (1.11), and the potential to inhibit nuclear factor-κB cascade to generate chemokine production manifested its utilization in the development of functional food attenuating inflammation-related disorders.

    Item Type: Article
    Uncontrolled Keywords: Crassostrea madrasensis; Polygalactosamino-glucopyranosyl; fucopyranose; Cyclooxygenase-2; Lipopolysaccharide-induced macrophages; Cytokines; Nuclear factor-κB
    Subjects: Biochemistry > Bioactive compounds
    Biochemistry
    Molluscan Fisheries > Bivalves
    Fish Biotechnology
    Divisions: CMFRI-Kochi > Marine Biotechnology
    Subject Area > CMFRI > CMFRI-Kochi > Marine Biotechnology
    CMFRI-Kochi > Marine Biotechnology
    Subject Area > CMFRI-Kochi > Marine Biotechnology
    Depositing User: Arun Surendran
    Date Deposited: 14 Aug 2020 06:26
    Last Modified: 14 Aug 2020 06:26
    URI: http://eprints.cmfri.org.in/id/eprint/14471

    Actions (login required)

    View Item View Item