Sulfated N-acetylglucosamino-glucuronopyranosyl-arabinopyranan from seafood Amphioctopus neglectus attenuates angiotensin-II prompted cardiac hypertrophy

Chakraborty, Kajal and Krishnan, Soumya and Joy, Minju (2020) Sulfated N-acetylglucosamino-glucuronopyranosyl-arabinopyranan from seafood Amphioctopus neglectus attenuates angiotensin-II prompted cardiac hypertrophy. International Journal of Biological Macromolecules, 163. pp. 1223-1232.

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    Abstract

    Angiotensin converting enzyme (ACE) is amultifunctional enzymeinvolved in translation of angiotensin-I (AngI) to vasoconstrictor angiotensin-II (AngII). A sulfated N-acetylglucosamino-glucuronopyranosyl-arabinopyranan characterized as poly-[(2-methoxy-β-arabinopyranosyl)-(1 → 3)-(β-glucurono)-(1 → 4)-(2-acetamido-2- deoxy-3,6-di-O-sulfonato-β-glucopyranose)] was purified and reported first time from the edible portion of Amphioctopus neglectus and evaluated for various pharmacological properties. The polysaccharide exhibited potential ACE attenuation property (IC50 0.11 mg mL−1), whereas molecular docking simulations displayed its efficient binding at the ACE active site with lesser inhibitory constant (Ki) of 17.36 nM and binding energy (−10.59 kcal mol−1). The in-vitro analysis showed that the studied polysacharide attenuated AngII prompted cardiac hypertrophy at 50 μg mL−1 in the cardiomyoblast cells, whereas 48% reduction in cellular surface area with extended viability could be correlated with anti-hypertrophic properties of the studied polysaccharide. The sulfated N-acetylglucosamino-glucuronopyranosyl-arabinopyranan purified fromA. neglectus could function as a prospective functional lead against the pathophysiological conditions leading to hypertension.

    Item Type: Article
    Uncontrolled Keywords: Amphioctopus neglectus; Sulfated N-acetylglucosaminoglucuronopyranosyl- arabinopyranan; Angiotensin-II induced cardiac hypertrophy
    Subjects: Biochemistry > Bioprospecting
    Biochemistry > Bioactive compounds
    Biochemistry
    Divisions: CMFRI-Kochi > Marine Biotechnology
    Subject Area > CMFRI > CMFRI-Kochi > Marine Biotechnology
    CMFRI-Kochi > Marine Biotechnology
    Subject Area > CMFRI-Kochi > Marine Biotechnology
    Depositing User: Arun Surendran
    Date Deposited: 20 Jul 2020 06:05
    Last Modified: 20 Jul 2020 08:25
    URI: http://eprints.cmfri.org.in/id/eprint/14393

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