Propensity of bioenrichment protocol in marine ornamental fish larviculture

Iyapparajanarasimapallavan, G (2015) Propensity of bioenrichment protocol in marine ornamental fish larviculture. Doctoral thesis, ICAR - Central Marine Fisheries Research Institute.


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    The Marine ornamentals are the most flourishing international business activity, valued at 8 billion US$ with an average annual growth rate of 9% across the world. Even though marine ornamental fish culture for trade is being practised globally, the early mortality of these altricial fish larvae in captivity are the major hitch of this sector that occurs due to lack of proper feed or feeding technology. This research is focused on the propensity of bio-enrichment protocol in marine ornamental fish larviculture using clownfish larvae (Amphiprion ocellaris) as a model species. Specifically, it was designed to optimize the feeding protocol of A. ocellaris using live feed enriched with DHA rich oil emulsion and to study their effects on larval growth and survival under captivity. The larvae of A. ocellaris were fed with six different diets including the indigenously produced DHA rich marine pelagic fish oil emulsion, which were delivered through rotifers Brachionus plicatilis. Locally available low-value oil sardine (Sardinella longiceps) and fish roe (egg) were screened for long chain n-3 PUFAs and were used as raw material to prepare the PUFA by chemical and/or enzymatic process. This study has been contemplated for the development of n-3 PUFA enriched formulation based on different physicochemical properties associated with the olefinic bonds in fatty acids and/or acyl chain length. The long chain C20-22n-3 PUFAs such as EPA and DHA present in the sardine oil were concentrated by saponification reaction followed by their enrichment by amide fractionation and chromatographic methods (the silica gel complexed with a d-block element). The unique substrate specificity of microbial triacylglycerol acyl hydrolases has also been utilized to further enrich the C20-22 LC PUFAs in triglycerides. The microalgal species were isolated from different locations of marine and backwater niches of Kerala, and their culture conditions in laboratory were optimized. The nutritional properties with respect to the fatty acid compositions of the purified microalgae at their exponential growth phase were obtained. The live feed rotifers were cultured in mass by using the shortlisted microalgae, and were further enriched with indigenously produced DHA rich fish oil emulsion to enhance the nutritional threshold of live feed. The optimum time period to enrich the live feed by the oil emulsion was described. The marine ornamental finfish A. ocellaris larvae was fed with the DHA enriched live feed rotifer in different experimental combinations, and the fatty acid compositions of the larvae were recorded to understand their effect on the growth and development of the fish larvae. The rotifers (Brachionus plicatilis) were mass cultured in the presence of DHA rich marine microalgae Isochrysis galbana and further enriched with DHA rich fish oil emulsion. The fatty acid profile along with the growth and survival of the enriched live fed were compared with those fed with different commercial diets including a lyophilized algal powder.

    Item Type: Thesis (Doctoral)
    Subjects: Biochemistry
    Aquaculture > Ornamental Fishes
    Divisions: CMFRI-Kochi > Marine Biotechnology
    Subject Area > CMFRI > CMFRI-Kochi > Marine Biotechnology
    CMFRI-Kochi > Marine Biotechnology
    Subject Area > CMFRI-Kochi > Marine Biotechnology
    Depositing User: Arun Surendran
    Date Deposited: 25 Jan 2020 04:33
    Last Modified: 25 Jan 2020 04:35

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