George, Githa Ann (2017) Establishment and characterisation of cell lines from the caerulean damsel, Pomacentrus caeruleus. Doctoral thesis, ICAR- Central Marine Fisheries Research Institute.
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Abstract
Cell lines and primary cell cultures from fish tissues have been used for research in disease diagnosis and cytotoxicity evaluation of environmental pollutants. The principal aim of this work was the development of continuous cell lines from the caerulean damsel, Pomacentus caeruleus. To achieve this, tissues were first sourced from donor fishes, devoid of contaminants using standardised disinfection protocols. Primary cultures were then initiated and suitable media, additives, dissociation reagents as well as optimum incubation temperature were determined. Successful primary cultures were subcultured and passaged to derive continuous cell lines which were cryopreserved for long term storage. The continuous cell lines established were characterised by immunotyping using cell type markers and authenticated to confirm the species of origin using previously established barcoding techniques. Preliminary applications such as gene transfection studies and cytotoxicity assays using bacterial extracellular products were done in addition to ensuring that the cultures and cryostocks were contamination-free.
| Item Type: | Thesis (Doctoral) |
|---|---|
| Uncontrolled Keywords: | cell lines; caerulean damsel; Pomacentrus caeruleus |
| Subjects: | Fish Biotechnology > Microbiology Fish Biotechnology |
| Divisions: | Contributors |
| Depositing User: | Arun Surendran |
| Date Deposited: | 01 Nov 2019 09:26 |
| Last Modified: | 30 Nov 2019 09:08 |
| URI: | http://eprints.cmfri.org.in/id/eprint/13915 |
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