First report of antioxidative abeo-oleanenes from red seaweed Gracilaria salicornia as dual inhibitors of starch digestive enzymes

Chakraborty, Kajal and Antony, Tima (2019) First report of antioxidative abeo-oleanenes from red seaweed Gracilaria salicornia as dual inhibitors of starch digestive enzymes. Medicinal Chemistry Research, 28 (5). pp. 696-710.

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Abstract

Carbolytic enzyme-associated cascades have been considered as potential curative target in attenuating diabetic mellitus pathogenesis. Two oleanene class of triterpenoids characterised as 24(4→23), 27(8→26), 30(20→29)-tris-abeo-olean-(12- oxo)-1,15,22-triene-methyl hept-5-enoate (1) and 24(4→23)-abeo-olean-(12-oxo)-3,5-diene-deconoate (2) with potential inhibitory activities against the starch digestive enzymes α-glucosidase and α-amylase, were purified from the organic extract of intertidal red seaweed Gracilaria salicornia (family Gracilariaceae). Structural interpretation of compounds was carried out by detailed spectroscopic analysis, and their antioxidant/anti-diabetic potentials were assessed. Inhibitory potential of abeo-oleanene derivative (2) towards the starch digestive enzymes, α-glucosidase (IC50 0.29 mM) and α-amylase (IC50 0.32 mM) were greater than those displayed by its abeo-oleanene chemotype 1 (IC50 0.34–0.40 mM). The molecular modelling studies were performed to designate the α-amylase and α-glucosidase inhibitory mechanism of oleanene analogues, and the comparison of docking parameters suggested that compound 2 exhibited least binding energy of −10.04 and −9.84 kcal mol−1 towards α-amylase and α-glucosidase respectively, and those results were corroborated with its greater inhibition potential against carbolytic enzymes. These results demonstrated that abeo-oleanene derivative (2) might constitute prospective anti-hyperglycaemic pharmaceutical candidate to moderate the likelihood of type-II diabetes.

Item Type:	Article
Uncontrolled Keywords:	Gracilaria salicornia; Gracilariaceae; Abeo-oleanenes; Anti-diabetic; Starch digestive enzymes; Biosynthetic pathway
Subjects:	Fish Biotechnology > Bioactive compound Marine Fisheries > Fish landing > New record Algae > Seaweed Algae
Divisions:	CMFRI-Kochi > Marine Biotechnology, Fish Nutrition and Health Division Subject Area > CMFRI > CMFRI-Kochi > Marine Biotechnology, Fish Nutrition and Health Division CMFRI-Kochi > Marine Biotechnology, Fish Nutrition and Health Division Subject Area > CMFRI-Kochi > Marine Biotechnology, Fish Nutrition and Health Division
Depositing User:	Arun Surendran
Date Deposited:	04 Apr 2019 05:43
Last Modified:	06 Dec 2022 05:57
URI:	http://eprints.cmfri.org.in/id/eprint/13614

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