Maintenance of haemocytes of edible oyster, Crassostrea madrasensis (Preston) in artificial media

Ittoop, Gijo and George, K C and Sanil, N K and Sobhana, K S and Nisha, P C (2001) Maintenance of haemocytes of edible oyster, Crassostrea madrasensis (Preston) in artificial media. Indian Journal of Fisheries, 48 (2). pp. 177-182.

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    Abstract

    The haemocytes of Crassostrea madrasensis were maintained in Hank's balanced salt solution (HBSS), HBSS with 10% foetal calf serum (FCS), tissue culture media M 199, and M 199 with 10% FCS. Total counts of viable haemocytes were taken at 0 hr, 24 hrs, 48 hrs, and 72 hrs. HBSS and HBSS with 10% FCS were found to maintain cell viability upto 72 hrs without much reduction in the count of viable cells. In M199 and M199 with 10% FCS, it was found that a substantial increase of small hyalinocytes occurred at 24 hrs and 48 hrs. This was followed by a steep fall in the number of viable cells at 72 hrs in both the media. The HBSS, HBSS with 10% FCS, M199 and M199 with 10% FCS were found to be good for maintaining the cells in viable state. However, only M 199 and M 199 with 10% FCS indicated multiplication of haemocytes.

    Item Type: Article
    Uncontrolled Keywords: Edible oyster;haemocytes;Crassostrea madrasensis
    Subjects: Molluscan Fisheries
    Fish Biotechnology
    Divisions: CMFRI-Kochi > Marine Biotechnology
    Subject Area > CMFRI Brochures > CMFRI-Kochi > Marine Biotechnology
    CMFRI-Kochi > Marine Biotechnology
    Depositing User: Dr. V Mohan
    Date Deposited: 12 Mar 2010 07:37
    Last Modified: 09 Sep 2015 15:05
    URI: http://eprints.cmfri.org.in/id/eprint/131

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