Ittoop, Gijo and George, K C and Sanil, N K and Sobhana, K S and Nisha, P C (2001) Maintenance of haemocytes of edible oyster, Crassostrea madrasensis (Preston) in artificial media. Indian Journal of Fisheries, 48 (2). pp. 177-182.
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Abstract
The haemocytes of Crassostrea madrasensis were maintained in Hank's balanced salt solution (HBSS), HBSS with 10% foetal calf serum (FCS), tissue culture media M 199, and M 199 with 10% FCS. Total counts of viable haemocytes were taken at 0 hr, 24 hrs, 48 hrs, and 72 hrs. HBSS and HBSS with 10% FCS were found to maintain cell viability upto 72 hrs without much reduction in the count of viable cells. In M199 and M199 with 10% FCS, it was found that a substantial increase of small hyalinocytes occurred at 24 hrs and 48 hrs. This was followed by a steep fall in the number of viable cells at 72 hrs in both the media. The HBSS, HBSS with 10% FCS, M199 and M199 with 10% FCS were found to be good for maintaining the cells in viable state. However, only M 199 and M 199 with 10% FCS indicated multiplication of haemocytes.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Edible oyster;haemocytes;Crassostrea madrasensis |
| Subjects: | Molluscan Fisheries Fish Biotechnology |
| Divisions: | CMFRI-Kochi > Marine Biotechnology, Fish Nutrition and Health Division Subject Area > CMFRI > CMFRI-Kochi > Marine Biotechnology, Fish Nutrition and Health Division CMFRI-Kochi > Marine Biotechnology, Fish Nutrition and Health Division Subject Area > CMFRI-Kochi > Marine Biotechnology, Fish Nutrition and Health Division |
| Depositing User: | Dr. V Mohan |
| Date Deposited: | 12 Mar 2010 07:37 |
| Last Modified: | 09 Sep 2015 15:05 |
| URI: | http://eprints.cmfri.org.in/id/eprint/131 |
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