Development and validation of microsatellite markers in a protandrous fish species Eleutheronema tetradactylum (Shaw, 1804) through cross-species amplification

Ismail, Shihab and Vineesh, N and Mohitha, C and Vijayagopal, P and Gopalakrishnan, A (2018) Development and validation of microsatellite markers in a protandrous fish species Eleutheronema tetradactylum (Shaw, 1804) through cross-species amplification. Indian Journal of Fisheries, 65 (3). pp. 47-51.

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    Abstract

    The four fingered threadfin Eleutheronema tetradactylum (Shaw, 1804) is a prioritised species for mariculture in India. Their demand in the domestic markets is rapidly growing. Genetic stock structure analysis of fish populations is an important aspect from fisheries management perspective. The present study was conducted to develop microsatellite primers through cross-priming to elucidate the genetic structure of E. tetradactylum. A total of 13 polymorphic microsatellite markers were developed from the resource species, Pacific salmon Polydactylus sexfilis. The observed mean and the effective number of alleles were found to be 11.962 and 6.927 respectively. The mean of observed heterozygosity (Ho) and expected heterozygosity (He) values obtained were 0.784 and 0.798 respectively. These new microsatellite markers can be used as effective tools for studying genetic disparity as well as for elucidating evolutionary relationships among E. tetradactylum populations.

    Item Type: Article
    Uncontrolled Keywords: Cross-priming, Eleutheronema tetradactylum, Indian waters, Polymorphic microsatellites
    Subjects: Fish and Fisheries > Fish Genetics
    Divisions: CMFRI-Kochi > Marine Biotechnology
    Subject Area > CMFRI > CMFRI-Kochi > Marine Biotechnology
    CMFRI-Kochi > Marine Biotechnology
    Subject Area > CMFRI-Kochi > Marine Biotechnology
    Depositing User: Arun Surendran
    Date Deposited: 03 Oct 2018 11:30
    Last Modified: 03 Oct 2018 11:30
    URI: http://eprints.cmfri.org.in/id/eprint/13038

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