Isolation and phylogenetic identification of heterotrophic thraustochytrids from mangrove habitats along the southwest coast of India and prospecting their PUFA accumulation

Jaseera, K V and Kaladharan, P and Vijayan, K K and Sandhya, S V and Leo Antony, M and Pradeep, M A Isolation and phylogenetic identification of heterotrophic thraustochytrids from mangrove habitats along the southwest coast of India and prospecting their PUFA accumulation. Journal of Applied Phycology. pp. 1-12.

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    Abstract

    Among several sustainable resources, marine heterotrophic microorganisms have gained much interest for various commercial applications owing to their potential to produce high quantities of polyunsaturated fatty acids. Above 70% of the globally produced fish oil is utilised for aqua feed formulations. Intense research is being carried out to find suitable alternatives to fish oil. Oleaginous protists are being explored as a promising substitute to fish oil and also for various high-value-added products. However, characterisation and utilisation of these unique protists remain underexplored in many countries including India and this prompted us to screen and isolate these heterotrophic microorganisms capable of producing PUFAs. In this study, 11 strains of heterotrophic thraustochytrid capable of producing significant quantities of polyunsaturated fatty acids were successfully isolated from different mangrove habitats along the southwest coast of India. Morphological characterisation along with molecular identification revealed that these strains showed close similarity to Aurantiochytrium spp. of the family Thraustochytridae. They are able to produce 7–11 g of dry biomass per litre of culture. Their fatty acid content was up to 12%(wt/wt) of biomass and GC analysis confirmed that these protists can accumulate docosahexaenoic acid as high as 22–60% of the total fatty acids. Their enzymatic activity, tolerance to various abiotic stress factors and antagonistic potential against vibrios are also described. These heterotrophic protists can antagonise aquaculture pathogens and act as an excellent source of docosahexaenoic acid that indicates their potential as a sustainable alternative to fish oil.

    Item Type: Article
    Uncontrolled Keywords: Aurantiochytrium spp.; PUFA; Thraustochytrids; Mangrove ecosystem; Fish oil alternative
    Subjects: Biochemistry > Bioactive compounds
    Marine Ecosystems > Mangroves
    Divisions: CMFRI-Kochi > Fishery Environment
    Subject Area > CMFRI > CMFRI-Kochi > Fishery Environment
    CMFRI-Kochi > Fishery Environment
    Subject Area > CMFRI-Kochi > Fishery Environment

    CMFRI-Kochi > Marine Biotechnology
    Subject Area > CMFRI > CMFRI-Kochi > Marine Biotechnology
    CMFRI-Kochi > Marine Biotechnology
    Subject Area > CMFRI-Kochi > Marine Biotechnology
    Depositing User: Arun Surendran
    Date Deposited: 28 Sep 2018 05:42
    Last Modified: 28 Sep 2018 05:42
    URI: http://eprints.cmfri.org.in/id/eprint/13035

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