Antioxidant, functional properties and amino acid composition of pepsin-derived protein hydrolysates from whole tilapia waste as influenced by pre-processing ice storage

Tejpal, C S and Vijayagopal, P and Elavarasan, K and Linga Prabu, D and Lekshmi, R G K and Asha, K K and Anandan, R and Chatterjee, N S and Mathew, S (2017) Antioxidant, functional properties and amino acid composition of pepsin-derived protein hydrolysates from whole tilapia waste as influenced by pre-processing ice storage. Journal of Food Science and Technology, 54 (13). pp. 4257-4267.

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Official URL: https://link.springer.com/article/10.1007/s13197-0...
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    Abstract

    t In recent years, hygienic handling of fishery waste is demanded owing to the fact that the fishery waste is an ideal raw material for the preparation of bioactive compounds. In the present study, the effect of pre-processing storage (at 4 ± 2 C) of whole tilapia waste (WTW) on the properties of its protein hydrolysate derived using pepsin was evaluated. Fish protein hydrolysates (FPH) were prepared from 0, 24 and 48 h old ice stored WTW and designated as FPH-0, FPH-1, and FPH-2, respectively. Total amino acids, total essential amino acids and total hydrophobic amino acids of FPH samples increased with the storage period of raw material (WTW). Antioxidant activities such as DPPH (2, 2 diphynyl-1- picrylhydrazyl) free radical scavenging activity and ferric reducing power of FPH samples were dose dependent. FPH-0 had better antioxidant properties including linoleic acid peroxidation inhibition activity than FPH-1 and FPH2. The DNA nicking assay revealed the protective effect of FPH preparations against Fenton’s reaction mediated oxidative damage. FPH-2 had better emulsifying properties and foaming stability whereas the FPH-0 had relatively good foaming capacity. SDS–PAGE indicated the presence of peptides ranging from 116 to\14.4 kDa in FPH-0 and less than 18 kDa in FPH-1 and FPH-2. The present study, clearly demonstrated that whole tilapia waste can effectively be converted to FPH and could be a potential ingredient in functional food and as a rich source of highquality protein in animal feed formulations.

    Item Type: Article
    Uncontrolled Keywords: Antioxidant; Amino acid composition; Functional properties; Bioactive fish protein hydrolysate; Tilapia fish waste; DNA nicking assay
    Subjects: Fish Biotechnology > Bioactive compound
    Food and Feeding
    Divisions: CMFRI-Kochi > Marine Biotechnology
    Subject Area > CMFRI > CMFRI-Kochi > Marine Biotechnology
    CMFRI-Kochi > Marine Biotechnology
    Subject Area > CMFRI-Kochi > Marine Biotechnology
    Depositing User: Arun Surendran
    Date Deposited: 23 Feb 2018 08:25
    Last Modified: 23 Feb 2018 08:25
    URI: http://eprints.cmfri.org.in/id/eprint/12636

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