Environmental DNA (eDNA) metabarcoding-based estimation of marine stocks

Jayasankar, P (2017) Environmental DNA (eDNA) metabarcoding-based estimation of marine stocks. In: Course Manual Summer School on Advanced Methods for Fish Stock Assessment and Fisheries Management. Lecture Note Series No. 2/2017 . CMFRI; Kochi, Kochi, pp. 317-319.

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    Information on species composition and biomass/abundance of exploited species in coastal fisheries is vital in management of resources. One of the most important mandates of the leading institution is judicious management of coastal and deep sea fishery resources. Traditional methods of identifying species and estimating biomass/abundance have inherent drawbacks which could be ameliorated by DNA marker based approach. Environmental DNA (eDNA) can be obtained from the skin, mucous, gamates, faeces, blood and other cells that are constantly being shed into the immediate environment by the organism. Analysis of this eDNA can give us information on the organisms, their abundance and biomass. Recent advances in next generation sequencing enable simultaneous sequencing of DNA from whole communities known as metabarcoding. Studies carried out in aquaria, large lakes, rivers and marine environment consistently suggest that eDNA metabarcoding outperforms traditional survey methods in terms of non-invasive sampling, sensitivity and cost incurred.

    Item Type: Book Section
    Uncontrolled Keywords: Environmental DNA; eDNA; metabarcoding-based estimation; marine stocks
    Subjects: Fish and Fisheries > Fish Genetics
    Divisions: CMFRI-Kochi > Marine Biotechnology
    Subject Area > CMFRI > CMFRI-Kochi > Marine Biotechnology
    CMFRI-Kochi > Marine Biotechnology
    Subject Area > CMFRI-Kochi > Marine Biotechnology
    Depositing User: Arun Surendran
    Date Deposited: 20 Sep 2017 08:57
    Last Modified: 20 Sep 2017 08:57
    URI: http://eprints.cmfri.org.in/id/eprint/12194

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