Development and characterization of two cell lines PDF and PDH from Puntius denisonii (Day 1865)

Lakra, W S and Goswami, Mukunda and Yadav, Kamalendra and Gopalakrishnan, A and Patiyal, R S and Sigh, M (2011) Development and characterization of two cell lines PDF and PDH from Puntius denisonii (Day 1865). In Vitro Cellular & Developmental Biology - Animal, 47. pp. 89-94. ISSN 1071-2690

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Official URL: https://www.ncbi.nlm.nih.gov/pubmed/21136193
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    Abstract

    The Puntius denisonii colloquially and more popularly referred to as Miss Kerala is a subtropical fish belonging to the genus Puntius (Barb) and family Cyprinidae. Two cell lines PDF and PDH were developed from the caudal fin and heart of P. denisonii, respectively. The cell lines were optimally maintained at 26°C in Leibovitz-15 medium supplemented with 10% fetal bovine serum. A diploid count of 50 chromosomes at passage 50 was observed in both the cell lines. The high growth potential of the cell lines was reflected from the cell doubling time of 28 and 30 h of PDF and PDH cell lines, respectively. The viability of the PDF and PDH cell lines was 70% and 76%, respectively, after 4 mo of storage in liquid nitrogen (−196°C). The origin of the cell lines was confirmed by the amplification of 653 bp fragments of cytochrome oxidase subunit I of mitochondrial DNA genes.

    Item Type: Article
    Uncontrolled Keywords: Puntius denisonii; PDF; PDH; Cell line
    Subjects: Aquaculture > Tropical fish
    Fish Genetics
    Divisions: Contributors
    Depositing User: Arun Surendran
    Date Deposited: 26 May 2017 10:33
    Last Modified: 26 May 2017 10:33
    URI: http://eprints.cmfri.org.in/id/eprint/11857

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