Fine structural changes of Rohu (Labeo rohita) sperm after dilution with cryoprotectants

Gopalakrishnan, A and Ponniah, A G and Lal, Kuldeep Kumar (2000) Fine structural changes of Rohu (Labeo rohita) sperm after dilution with cryoprotectants. Indian Journal of Fisheries, 47 (1). pp. 21-27.

AGKN Fine structural changes of Rohu Labeo rohita sperm_Indian Journal of Fisheries_47_1_2000.pdf

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    Spermatozoa of rohu (Labeo rohita) were diluted in two cryodiluents and fine structural changes were investigated. Damages were observed in head, mid-piece and tail of about 51% spermatozoa immediately after dilution in one of the cryodiluents. After 10 minutes of equilibration period, the frequency of damaged sperms increased to 85%. The other diluent did not exhibit signif~cant structural changes unlike the former one and nearly 60% sperms remained visibly intact even after 10 minutes of equilibration time. The present investigation points out (1) diluents inhibiting sperm motility alone need not always be ideal for a species and (2) usefulness of ultrastructural images of fish sperms in determining the fertilizing ability of milt and in preliminary screening and selection of cryodiluents for deepfreezing programmes.

    Item Type: Article
    Uncontrolled Keywords: Fine structural changes; Rohu (Labeo rohita); dilution with cryoprotectants
    Subjects: Fish Genetics
    Divisions: Contributors
    Depositing User: Arun Surendran
    Date Deposited: 20 May 2017 10:19
    Last Modified: 20 May 2017 10:19

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