Genetic variation and phylogenetic relationship between two species of yellow catfish, Horabagrus brachysoma and H. nigricollaris (Teleostei: Horabagridae) based on RAPD and microsatellite markers

Abdul Muneer, P M and Gopalakrishnan, A and Shivanandan, Remya and Basheer, V S and Ponniah, A G (2011) Genetic variation and phylogenetic relationship between two species of yellow catfish, Horabagrus brachysoma and H. nigricollaris (Teleostei: Horabagridae) based on RAPD and microsatellite markers. Molecular Biology Reports, 38 (4). pp. 2225-2232.

[img] Text
Genetic variation and phylogenetic relationship_AGKN Muneer et al. Mol Biol Rep 2011_38-4.pdf
Restricted to Registered users only

Download (367kB) | Request a copy
Official URL: https://www.ncbi.nlm.nih.gov/pubmed/20862549
Related URLs:

    Abstract

    The two species of yellow catfish, Horabagrus brachysoma and H. nigricollaris are categorized as ‘endangered’ and ‘critically endangered’ respectively in their wild habitat. Proper knowledge of genetic structure and variability of these endangered species are highly essential for the management, conservation and improvement of fish stocks. Therefore, genetic variation and phylogenetic relationships between these species of yellow catfish sampled from Chalakkudy River in the hot spot of biodiversity- Western Ghats region, Kerala, India were analyzed by using Random amplified polymorphic DNA (RAPD) and microsatellite markers. 85 RAPD and five microsatellites loci were detected to analyze the genetic variation and phylogenetic relationships among these species. Out of 85 RAPD loci produced only 52.94% were polymorphic whereas in microsatellite, all 5 loci were polymorphic (100%). Species specific RAPD bands were found in both species studied. In microsatellite, the number of alleles across the five loci ranged from 1 to 8. The observed heterozygosities in H. brachysoma and H. nigricollaris were 0.463 and 0.443, respectively. Here, both RAPD and microsatellite methods reported a low degree of gene diversity and lack of genetic heterogeneity in both species of Horabagrus which strongly emphasize the need of fishery management, conservation and rehabilitation of these species.

    Item Type: Article
    Uncontrolled Keywords: Horabagrus; Phylogeny; RAPD; Genetic variation; Microsatellites; Polymorphism
    Subjects: Fish Genetics
    Divisions: Contributors
    Depositing User: Arun Surendran
    Date Deposited: 20 May 2017 09:28
    Last Modified: 20 May 2017 09:28
    URI: http://eprints.cmfri.org.in/id/eprint/11787

    Actions (login required)

    View Item View Item