Nuclear and mitochondrial DNA markers based identification of blunthorn lobster Palinustus waguensis Kubo, 1963 from South-west coast of India

Chakraborty, Rekha D and Maheswarudu, G and Purushothaman, P and Kuberan, G and Sebastian, Jomon and Radhakrishnan, E V and Thangaraja, R (2016) Nuclear and mitochondrial DNA markers based identification of blunthorn lobster Palinustus waguensis Kubo, 1963 from South-west coast of India. Indian Journal of Biotechnology, 15. pp. 172-177.

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    Abstract

    The present study reports the occurrence of Palinustus waguensis Kubo, 1963 from South-west coast of India on the basis of morphological as well as molecular data. The DNA sequences of two mitochondrial genes, large subunit ribosomal DNA (16S rDNA) and cytochrome oxidase I (COI), and two nuclear-protein coding genes, viz., phosphoenolpyruvate carboxykinase (PEPCK) and sodium-potassium ATPase α-subunit (NaK), were submitted in the GenBank. Morphological examination of the specimen confirmed the identity of the species as P. waguensis. The pairwise genetic distance analysis, sequence identity and phylogenetic tree have shown that the specimen has similarity only with 16S rDNA sequence of P. waguensis of earlier reports. In the case of other genetic markers, it has shown some identity towards P. unicornutus and P. holthuisi, but with a high genetic difference. This study with sequence data of P. waguensis in GenBank will help to identify the specimen on molecular basis in future.

    Item Type: Article
    Uncontrolled Keywords: Blunthorn lobster (Palinustus waguensis), deepsea, genetic markers, phylogenetics, taxonomy
    Subjects: Fish Biotechnology
    Crustacean Fisheries > Lobsters
    Fish Genetics
    Divisions: CMFRI-Kochi > Marine Capture > Crustacean Fisheries
    Subject Area > CMFRI > CMFRI-Kochi > Marine Capture > Crustacean Fisheries
    CMFRI-Kochi > Marine Capture > Crustacean Fisheries
    Subject Area > CMFRI-Kochi > Marine Capture > Crustacean Fisheries
    Depositing User: Arun Surendran
    Date Deposited: 07 Dec 2016 09:12
    Last Modified: 07 Dec 2016 09:12
    URI: http://eprints.cmfri.org.in/id/eprint/11313

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