Effect of salinity stress on biochemical constituents and ArHsp22 gene expression in Artemia franciscana

Vikas, P A and Thomas, P C and Sajesh Kumar, N K and Chakraborty, Kajal and Sanil, N K and Vijayan, K K (2016) Effect of salinity stress on biochemical constituents and ArHsp22 gene expression in Artemia franciscana. Indian Journal of Fisheries, 61 (3). pp. 150-156.

[img]
Preview
Text
23.50894.pdf

Download (765kB) | Preview
Related URLs:

    Abstract

    The present study analysed the effect of salinity stress on survival, biochemical constituents such as soluble protein, amino acid, trehalose and fatty acid, as well as real time expression of the Artemia heat-shock protein 22 (ArHsp 22) gene in Artemia franciscana. Results of the study revealed that Artemia can withstand sudden salinity increase up to 200 ppt without any mortality up to 6 h. Significantly higher mortality percentage was recorded at 24 h of incubation at 200 ppt. Short-term exposure to hypersaline conditions significantly reduced the protein content in Artemia while the protein biosynthesis enhanced after 24 h of incubation at 150 ppt salinity. Long term exposure to 200 ppt salinity did not show any further increase in amino acid content as observed at 100 and 150 ppt conditions, indicating reduced rate of amino acid metabolism and stress. Salt stress induced the synthesis of total polyunsaturated fatty acids (PUFA) in Artemia especially the 22:6n-3 and 20:5n-3 after 24 h of incubation at 150 ppt . Exposure to higher salinity induced trehalose production which indicated its vital role in combating salt stress in Artemia. The present study also indicated that short-term salt stress can significantly enhance the ArHsp22 gene expression in Artemia adults.

    Item Type: Article
    Uncontrolled Keywords: Amino acid, ArHsp22 gene, Carotenoid, Fatty acid, Salt stress, Trehalose
    Subjects: Crustacean Fisheries > Artemia
    Biochemistry > Biochemical composition
    Fish and Fisheries > Biochemical Study
    Divisions: CMFRI-Kochi > Marine Biotechnology
    Subject Area > CMFRI > CMFRI-Kochi > Marine Biotechnology
    CMFRI-Kochi > Marine Biotechnology
    Subject Area > CMFRI-Kochi > Marine Biotechnology
    Depositing User: Arun Surendran
    Date Deposited: 22 Oct 2016 10:07
    Last Modified: 22 Oct 2016 10:07
    URI: http://eprints.cmfri.org.in/id/eprint/11219

    Actions (login required)

    View Item View Item