Cryodiluents and morphological changes in the spermatozoa of fish Liza parsia (Hamilton- Buchanan).

Sukumaran, Sandhya (2001) Cryodiluents and morphological changes in the spermatozoa of fish Liza parsia (Hamilton- Buchanan). Masters thesis, Central Institute of Fisheries Education.

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    Abstract

    An attempt has been made to get an insight into cryoinjuries that occur during the process o f cryopreservation and to identify the best cryodiluent for the milt of Liza parsia, one of the euryhaline fishes of immense culture potential. The morphological changes or damages caused by the cryodiluent at various stages of sperm preservation in liquid nitrogen at -196°C were studied at ultrastructural level by Transmission Electron Microscopy (TEM). V2E+DMSO diluent accorded maximum protection to the spermatozoa and inflicted least damages, retaining maximum post-thaw motility.Hence, V2E+DMS0 appear to be the best cryodiluent for the milt of Liza parsia. The present findings need further confirmation with actual fertility trials.

    Item Type: Thesis (Masters)
    Uncontrolled Keywords: Cryodiluents; Morphological changes; Spermatozoa; Fish; Liza parsia (Hamilton- Buchanan)
    Subjects: Dissertations
    Demersal Fishes > Mullet
    Divisions: CMFRI-Kochi > Mariculture
    Subject Area > CMFRI > CMFRI-Kochi > Mariculture
    CMFRI-Kochi > Mariculture
    Subject Area > CMFRI-Kochi > Mariculture
    Depositing User: Mr Rakesh
    Date Deposited: 17 Sep 2016 04:34
    Last Modified: 06 Oct 2016 10:48
    URI: http://eprints.cmfri.org.in/id/eprint/11127

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