Effect of a putative probiotic bacterial isolate from shrimp gut on disease resistance and immune response in shrimp.

Mary Sophia, J (2004) Effect of a putative probiotic bacterial isolate from shrimp gut on disease resistance and immune response in shrimp. Masters thesis, Central Institute of Fisheries Education.

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    Abstract

    Twenty two bacterial isolates from healthy juveniles of Fenneropenaeus indicus were tested for their suitability to be used as gut probionts. Out of these, two strains viz., G23 and V76 from F.indicus gut and hemolymph respectively, showed good antagonism towards all the bacterial pathogens used for testing. Two trials were conducted to test the efficacy o f the selected probionts on growth, disease resistance and/ immune parameters in shrimp. In trial 1, postlarvae of P. monodon were fed on commercial shrimp diets incorporated with the selected probionts G23 , V76 and an unaltered diet for a period of 45 days. On termination o f the experimental feeding, the treatment groups fed on G23 incorporated diet showed significantly (P< 0.05) higher growth and disease resistance to Vibrio harveyi. There was also good colonization of the probiont in the gut and significant reduction in vibrio load in the gut of challenged animals (with V. harveyi) on the 10th day post challenge compared to the control groups. Groups fed on V76 incorporated diets also showed comparatively better performance than control group, but was not as significant as in the case of G23. In the second trial, juveniles of F.indicus were fed on G23 incorporated diet for a period of 45 days along with control groups fed on unaltered diet. The groups fed on G23 incorporated diet showed significant (P< 0.05) improvement in disease resistance to Vibrio anguillarum. The effect of the diets on the immune parameters like total and differential hemocyte counts, plasma protein, plasma bactericidal activity, phenoloxidase and respiratory burst activity In the hemocytes were also tested. It was found that the plasma bactericidal activity and the percentage composition of granulocytes in the hemolymph were significantly higher in the G23 supplemented groups as compared to control animals. The groups supplemented with G23 also showed good colonization of the probiont in the gut and significant reduction in total vibrio count in the gut of challenged animals on 10th day post challenge with Vibrio anguillarum.

    Item Type: Thesis (Masters)
    Uncontrolled Keywords: Putative probiotic bacteria; Shrimp gut; Disease resistance; Immune response; Shrimp
    Subjects: Dissertations
    Crustacean Fisheries > Prawn and Prawn fisheries
    Fish Diseases
    Divisions: CMFRI-Kochi > Mariculture
    Subject Area > CMFRI > CMFRI-Kochi > Mariculture
    CMFRI-Kochi > Mariculture
    Subject Area > CMFRI-Kochi > Mariculture
    Depositing User: Mr Rakesh
    Date Deposited: 08 Sep 2016 10:39
    Last Modified: 07 Oct 2016 04:14
    URI: http://eprints.cmfri.org.in/id/eprint/11085

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