High density culture of marine microalgae using semi-continuous and continuous systems.

Bharguram, Lambade Sunil (1999) High density culture of marine microalgae using semi-continuous and continuous systems. Masters thesis, Central Institute of Fisheries Education.

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    Abstract

    This study entitled "High density culture o f marine micro-algae using semicontinuous and continuous systems" was carried out with the objectives of devising small-scale low cost systems for use in laboratory conditions. The study was carried out using two commonly used species of microalgae viz., the flagellate Isochrysis galbana and the diatom, Chaetoceros sp. The system devised was a modified turbidostat (2 1 to 5 1) having three tier gravitational flow with manual controllers and without automation. For scale-up semicontinuous culture, a 60 1 capacity internally illuminated plexy glass culture system was devised. Using 2.5 times the normal nutrient concentration, limited supply o f CO2 , activated charcoal filtered air and under sterile conditions it was possible to obtain upto 5 fold increase in culture cell density when compared to the normal batch cultures. Besides it was possible to maintain the cultures for durations varying from 2 to 5 weeks without collapse. In the continuous culture system o f 2 I capacity . The daily harvest o f high density culture amounted to 500 ml, and in the semi-continuous system (4 1 capacity). The once-in-3 day harvest varied between 2-3 litres. From a single 60 1 capacity semicontinuous system running for 36 days the total larvest amounted to 530 litres o f average 3.2 million cells/ml culture. With respect to maricuhure, the application of conclusions derived from present.work would resut in increased efficiency and reduced cost of production of microalgae.

    Item Type: Thesis (Masters)
    Uncontrolled Keywords: High density culture; Marine microalgae; Semi-continuous and continuous systems
    Subjects: Dissertations
    Algae
    Divisions: CMFRI-Kochi > Mariculture
    Subject Area > CMFRI > CMFRI-Kochi > Mariculture
    CMFRI-Kochi > Mariculture
    Subject Area > CMFRI-Kochi > Mariculture
    Depositing User: Mr Rakesh
    Date Deposited: 08 Sep 2016 06:53
    Last Modified: 07 Oct 2016 04:17
    URI: http://eprints.cmfri.org.in/id/eprint/11077

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