Extra chromosomal DNA diversity and drug resistance in aeromonas hydrophila of aquaculture systems.

Halawai, Brijesh Kumar (2003) Extra chromosomal DNA diversity and drug resistance in aeromonas hydrophila of aquaculture systems. Masters thesis, Central Institute of Fisheries Education.

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    Abstract

    Twenty-two isolates of Aeromonas hydrophila collected from fish, water and sediment samples of aquaculture systems were screened for their plasmid DNA and protein profiles as well as susceptibility to twenty antimicrobial agents. The plasmid occurrence rate was 100% and a 21 Kilo base plasmid was common to all isolates. The number of plasmid ranged from 1 to 4 while size ranged from 1 to 21 Kilo base. The eight different types of plasmid profiles among these isolates reflected diversity of extrachromosomal DNA. The most common plasmid profile was a single banded of 21 Kilo base exhibited by 50% of the isolates All the isolates showed multiple resistance patterns ranging from resistance to eleven to fourteen antibiotics. All the isolates were resistant to ampicillin, amoxycillin, cephalexin, vancomycin, bacitracin, penicillin-G and lincomycin, while all were sensitive to ofloxacin, nitrofurazone, gentamycin, chloramphenicol and pefloxacin. Cellular protein profile resolved through NATIVE-PAGE and SDS-PAGE also revealed heterogeneity among the isolates. While some of the protein bands were shared by ail isolates, others were varied in the isolates. Random primed PCR amplification of the plasmid DNA from different plasmid profile groups was also carried out to generate RARD profile. While some of the amplicons were species specific, produced by all the isolates, others were discriminatory. The RAPD analysis revealed high level of plasmid DNA diversity among the isolates, which were not evident in simple plasmid profiling, making it good tool for evaluation of extrachromosomal diversity and epidemiological studies of A. hydrophila. Intraspecies diversity of extrachromosomal DNA of A. hydrophila from aquaculture systems was indicated from plasmid profile, antibiogram, cellular protein profile and RAPD pattern of plasmid DNA.

    Item Type: Thesis (Masters)
    Uncontrolled Keywords: Chromosomal DNA diversity; Drug resistance; Aeromonas hydrophila; Aquaculture systems
    Subjects: Dissertations
    Aquaculture
    Fish Diseases
    Divisions: CMFRI-Kochi > Mariculture
    Subject Area > CMFRI > CMFRI-Kochi > Mariculture
    CMFRI-Kochi > Mariculture
    Subject Area > CMFRI-Kochi > Mariculture
    Depositing User: Mr Rakesh
    Date Deposited: 06 Sep 2016 04:38
    Last Modified: 07 Oct 2016 04:26
    URI: http://eprints.cmfri.org.in/id/eprint/11042

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