Electrophoretic patterns of the general proteins of four species belonging to the Family Carangidae.

Verghese, Bindhu (1998) Electrophoretic patterns of the general proteins of four species belonging to the Family Carangidae. Masters thesis, Central Institute of Fisheries Education.

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    Abstract

    Muscle proteins usually provide useful information and therefore are frequently used as valuable diagnostic characters in the classification of fishes at species, generic, familial and higher taxonomic levels. The muscle myogen of four species of carangids. viz, Decapterus russelli, D. macrosoma, Selar crumenophthalmus and Megalaspis cordyla using horizontal slab polyacrylamide gel electrophoresis (Gel conc. 7.5%) is reported in the present communication. The number of protein fractions and the presumptive loci in muscle of D. russelli, D. macrosoma, S. crumenophthalmus and M. cordyla were found to be 9,7,6 and 5 respectively. The Rf value ranged from 21 to 84 for S. crumenophthalmus, 2 to 72 for M cordyla, 15 to 89 for D. macrosoma and 18 to 96 for D. russelli. The difference in number of fractions, their mobility pattern and staining intensity indicated species-specificity. With the limited number of samples used in the present work, there has been an indication of intraspecific polymorphism among three species of carangids, being 3.5% in D. russelli, 13.5% in D. macrosoma and 6.6% in S. crumenophthalmus. In M. cordyla no polymorphism was observed. This work also revealed that protein fractions in carangids can be used as species - specific markers, which could be helpful in resolving disputes in the event of any taxonomic ambiguity.

    Item Type: Thesis (Masters)
    Uncontrolled Keywords: Electrophoretic patterns; General proteins; Family Carangidae
    Subjects: Dissertations
    Fish Biotechnology
    Divisions: CMFRI-Kochi > Mariculture
    Subject Area > CMFRI > CMFRI-Kochi > Mariculture
    CMFRI-Kochi > Mariculture
    Subject Area > CMFRI-Kochi > Mariculture
    Depositing User: Mr Rakesh
    Date Deposited: 05 Sep 2016 09:58
    Last Modified: 07 Oct 2016 04:27
    URI: http://eprints.cmfri.org.in/id/eprint/11038

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