A cell culture system developed from heart tissue of the greasy grouper, Epinephelus tauvina (Forsskal 1775) by enzymatic dissociation

Sobhana, K S and George, K C and Venkat Ravi, G and Ittoop, Gijo and Paulraj, R (2008) A cell culture system developed from heart tissue of the greasy grouper, Epinephelus tauvina (Forsskal 1775) by enzymatic dissociation. Indian Journal of Fisheries, 55 (3). pp. 251-256.

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Abstract

Heart tissue samples aseptically excised from healthy juveniles of the grouper, Epinephelus tauvina were subjected to enzymatic dissociation using 0.25 % trypsin solution, under aseptic conditions. The resultant cells were suspended in Leibovitz' L-15 medium supplemented with 20 % fetal bovine serum. The cells were subsequently seeded into 25 cm2 tissue culture flasks at a density of 106 ml-1 and incubated at 28 ± 2°C. Cells showed spreading and attachment to the culture flasks within 24 h and formed monolayers comprising of epithelioid as well as fibroblast-like cells within 20 days. A confluent monolayer filling the flask surface comprising predominantly fibroblastic cells was formed within 30 to 35 days. The cell monolayer was harvested for passage by trypsinisation, which formed successful monolayers in subsequent subcultures.

Item Type:Article
Uncontrolled Keywords:greasy grouper; Epinephelus tauvina
Subjects:Demersal Fishes > Groupers
Divisions:CMFRI-Cochin > Marine Biotechnology
ID Code:110
Deposited By:Edwin Joseph
Deposited On:11 Mar 2010 14:58
Last Modified:11 May 2012 15:07

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