DNA barcoding of gobiid fishes (Perciformes, Gobioidei)

Viswambharan, Divya and Pavan-Kumar, A and Singh, Dhirendra P and Jaiswar, A K and Chakraborty, S K and Nair, J Rajasekharan and Lakra, W S (2015) DNA barcoding of gobiid fishes (Perciformes, Gobioidei). Mitochondrial DNA, 26 (1). pp. 15-19.

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    Abstract

    Gobiids constitute a major proportion of fish population in both tropical and temperate freshwater as well as marine ecosystem. Due to their small size, cryptic ecology and ambiguous morphological characters, gobiids diversity was not documented completely. In this study, DNA barcodes were generated for 11 species of gobiids, collected from the Ashtamudi Lake, India. The mitochondrial COI gene was amplified using universal primers and the resulted 650 bp amplicon was sequenced. The COI barcodes clearly distinguished all the species with high interspecific genetic distance values than intra-specific values based on K2P (Kimura 2 Parameter) model. The average genetic distance (K2P model) within species, genus and family was 1.2%, 22.2% and 25.3%, respectively. In addition to barcode-based species identification system, Nucleotide Diagnostic (ND) characters specific for species were identified. The Neighbor-Joining tree revealed distinct clusters shared by the species of same genera.

    Item Type: Article
    Uncontrolled Keywords: Cytochrome c oxidase subunit I, DNA barcoding, gobiid fishes, nucleotide diagnostic characters
    Subjects: Fish Genetics
    Divisions: CMFRI-Mangalore
    Depositing User: Arun Surendran
    Date Deposited: 23 Jun 2016 08:07
    Last Modified: 03 Feb 2017 05:50
    URI: http://eprints.cmfri.org.in/id/eprint/10866

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